Characterization of Oral Immunity in Cases and Close Household Contacts Exposed to Andes Orthohantavirus (ANDV).
Characterization of Oral Immunity in Cases and Close Household Contacts Exposed to Andes Orthohantavirus (ANDV).
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DOI：
10.3389/fcimb.2020.557273
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发表时间：
2020
影响因子：
5.7
通讯作者：
Le Corre N
中科院分区：
文献类型：
--
作者：
Martinez-Valdebenito C;Andaur C;Angulo J;Henriquez C;Ferrés M;Le Corre N
背景：安第斯正汉坦病毒（ANDV）是智利汉坦病毒心肺综合征的唯一病原，也是迄今为止已知的唯一通过人际传播的汉坦病毒。人与人之间传播的主要风险是性伴侣的指示性病例，而深吻是感染的主要机制。实验报告表明，一些唾液成分可以抑制ANDV感染。因此，一些宿主因素，如唾液质量，可以帮助解释为什么有些人即使暴露在病毒中也不会被感染。目的：比较一些唾液成分，如细胞因子和粘蛋白，在andv感染病例（暴露-患病），他们的密切家庭接触者（暴露-未患病）和健康对照未暴露。方法：69例ANDV确诊病例，76例家庭密切接触者（CHC）和39例未暴露健康对照（HCNE）。测定唾液中下列成分：ELISA法测定分泌性免疫球蛋白A (sIgA)；细胞因子（IL1β、IL12p70、TNFα、INFy、IL10、IL6、VEGF、IP10、IL8）多重检测，黏素MUC7、MUC5B免疫印迹检测。结果：在感染病例中，男性占74%，男性占45%，男性占33% （p≤0.05）。病例、CHC和HCNE的平均年龄分别为37.7岁、28.7岁和32岁（p≤0.05）。感染组sIgA平均浓度为4.846 mg/mL，高于CHC组的0.333 mg/mL （p≤0.05）。对于细胞因子，各组间IFNy、IL12p70和IL8的表达差异显著。CHC组MUC7亚型检出率（62.6%,31/49）高于andv组（40.5%,17/42）（p < 0.05）。CHC组MUC5B阳性率（62.16%,46/74）高于andv感染组（44.4%,28/63）（p < 0.05）。结论：三种唾液成分在感染病例和家庭密切接触者之间存在差异（sIgA、细胞因子和粘蛋白）。这些差异可以用andv感染病例组中疾病的急性状态来解释。然而，MUC5B和MUC7亚型的差异并不能完全用感染本身来解释。这项工作代表了在ANDV感染的背景下唾液成分的新描述。
Background: Andes orthohantavirus (ANDV) is the sole etiologic agent of Hantavirus Cardiopulmonary Syndrome in Chile and, until now, the only Hantavirus known to be transmitted by person-to-person route. The main risk of person-to-person transmission is to be a sexual partner of an index case, and deep kissing the main mechanism of infection. Experimental reports suggest that ANDV infection can be inhibited by some saliva components. Therefore, some host factors like saliva quality, could help to explain why some individuals do not become infected even though their exposure to the virus is high. Aim: To compare some saliva components, such cytokines and mucins, between ANDV-infected cases (exposed-sick), their close household contacts (exposed-not sick) and healthy control not exposed. Methods: Sixty-nine confirmed ANDV-infected cases, 76 close household contacts exposed to ANDV but not infected (CHC) and 39 healthy control not exposed (HCNE). The following components were measured in saliva: secretory immunoglobulin A (sIgA) by ELISA; cytokines (IL1β, IL12p70, TNFα, INFy, IL10, IL6, VEGF, IP10, and IL8) by Multiplex Assay and mucins MUC7 and MUC5B by Western Blotting. Results: Among infected cases, CHC and HCNE analyzed 74, 45, and 33% were men, respectively (p ≤ 0.05). The average age for cases, CHC and HCNE was 37.7, 28.7, and 32 years, respectively (p ≤ 0.05). The average concentration of sIgA in infected cases was 4.846 mg/mL, higher than for CHC group, 0.333 mg/mL (p ≤ 0.05). For cytokines, significant differences were found comparing all groups for IFNy, IL12p70, and IL8. Among CHC group, there was a higher frequency of detection of MUC7 isoform (62.6%; 31/49) compared to ANDV-infected cases (40.5%; 17/42) (p < 0.05). Similarly, presence of MUC5B was higher among CHC groups (62.16%; 46/74) than in ANDV-infected cases (44.4%; 28/63) (p < 0.05). Conclusions: Three salivary components showed differences between infected cases and close household contacts (sIgA, cytokines, and mucins). These differences can be explained by the acute state of the disease in the ANDV-infected cases group. However, the differences in MUC5B and isoforms of MUC7 are not entirely explainable by the infection itself. This work represents a novel description of salivary components in the context of ANDV infection.