Spermatozoa of the Atlantic bottlenosed dolphin, Tursiops truncatus.
Spermatozoa of the Atlantic bottlenosed dolphin, Tursiops truncatus.
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DOI：
10.1530/jrf.0.0630509
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发表时间：
1981-11
期刊：
影响因子：
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通讯作者：
A. D. Fleming;R. Yanagimachi;H. Yanagimachi
中科院分区：
文献类型：
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作者：
A. D. Fleming;R. Yanagimachi;H. Yanagimachi
一只圈养的雄性海豚的精液是通过电射精收集的，并将其冷冻到零下176摄氏度，10天后解冻后精子活力良好。电子显微镜显示顶体后区有14-16个平行的脊线，中段有两种类型的线粒体。精子在预培养2 h后才能与去透明带仓鼠卵子融合，提示在受精前需要精子获能和顶体反应。
Semen from a male dolphin in captivity was collected by electro-ejaculation and frozen to -176 degrees C. Sperm motility was excellent after thawing 10 days later. Electron microscopy showed 14-16 parallel ridges in the post-acrosomal region and two types of mitochondria in the mid-piece. The spermatozoa were capable of fusing with zona-free hamster eggs only after preincubation for 2 h, suggesting the need for sperm capacitation and acrosome reaction before fertilization in this species.