Pathogenesis of of cytomegalovirus infection. I. Activation of virus from bone marrow-derived lymphocytes by in vitro allogenic reaction.
Pathogenesis of of cytomegalovirus infection. I. Activation of virus from bone marrow-derived lymphocytes by in vitro allogenic reaction.
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DOI：
10.1084/jem.141.3.561
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发表时间：
1975-03-01
期刊：
影响因子：
--
通讯作者：
Oldstone MB
中科院分区：
文献类型：
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作者：
Olding LB;Jensen FC;Oldstone MB
在子宫内或出生时感染细胞培养适应的小鼠巨细胞病毒（MCMV）株后，一些小鼠株在存在特异性抗病毒抗体的情况下发生潜伏病毒感染。感染后5个月，MCMV可以从感染动物的脾脏淋巴细胞中被激活并恢复，这些细胞与来自未感染动物的组织不相容（H-2外源）小鼠胚胎细胞共培养。相比之下，将感染小鼠的淋巴样细胞与来自同基因、组织相容（H-2相似）供体的小鼠胚胎细胞共同培养不会激活MCMV。同样，MCMV不能从超声淋巴样细胞中恢复。用脂多糖（一种b细胞有丝分裂原）处理活的淋巴样细胞可激活病毒，但其他多种有丝分裂原如植物血凝素、豆豆蛋白a或美洲商陆有丝分裂原不能激活病毒。随后用各种技术从感染小鼠的淋巴样细胞中纯化MCMV，表明MCMV在b淋巴细胞群中存在。
After infection in utero or at birth with a cell culture adapted strain of mouse cytomegalovirus (MCMV), several mouse strains developed a latent virus infection in the presence of specific antiviral antibodies. Up to 5 mo after infection, MCMV could be activated and recovered from spleen lymphocytes of the infected animals that were co- cultivated with histoincompatible (H-2 foreign) mouse embryo cells from uninfected animals. In contrast, co-cultivation of lymphoid cells from infected mice with mouse embryo cells from syngeneic, histocompatible (H-2 similar) donors did not activate MCMV. Similarly, MCMV was not recovered from sonicated lymphoid cells. Virus was activated by treating viable lymphoid cells with lipopolysaccharide, a B-cell mitogen, but was not activated by a variety of other mitogens such as phytohemagglutinin, concanavalin A, or pokeweed mitogen. Subsequent purification of lymphoid cells from the infected mice by a variety of techniques indicated that MCMV was harbored in the B-lymphocyte population.