Contribution of treated wastewater to the contamination of recreational river areas with Cryptosporidium spp. and Giardia duodenalis
Contribution of treated wastewater to the contamination of recreational river areas with Cryptosporidium spp. and Giardia duodenalis
复制标题
DOI：
10.1016/j.watres.2008.05.001
复制
发表时间：
2008-07-01
期刊：
影响因子：
12.8
通讯作者：
Mezo, Mercedes
中科院分区：
文献类型：
--
作者：
Antonio Castro-Hermida, Jose;Garcia-Presedo, Ignacio;Mezo, Mercedes
对加利西亚（西班牙西北部）12 个废水处理厂的进水和最终出水样本进行了分析，以确定是否存在隐孢子虫。卵囊和十二指肠贾第鞭毛虫囊肿。所有工厂都将污水排放到水文盆地，该盆地有许多休闲区和河滩。样本（25-50 升）于 2007 年春季、夏季、秋季和冬季收集。使用美国环境保护署方法 1623 中包含的技术对总共 96 个样本进行了分析。为了鉴定存在的基因型，对以下基因进行了扩增和测序：18S SSU rRNA（隐孢子虫属）和 β-贾第虫（G. duodenalis）。全年所有处理厂的进水和出水样本中均检测到这两种寄生虫（100%），并且十二指肠球菌的数量始终超过隐孢子虫属。每升进水中十二指肠球菌的平均浓度明显较高（P
Samples of the influent and final effluent from 12 wastewater treatment plants from Galicia (NW, Spain) were analyzed for the presence of Cryptosporidium spp. oocysts and Giardia duodenalis cysts. All of the plants discharge effluent to a hydrographic basin in which there are numerous recreational areas and fluvial beaches. The samples (25-50 liters) were collected in spring, summer, autumn and winter of 2007. A total of 96 samples were analyzed using techniques included in the US Environmental Protection Agency Method 1623. To identify the genotypes present, the following genes were amplified and sequenced: 18S SSU rRNA (Cryptosporidium spp.) and beta-giardina (G. duodenalis). Both parasites were detected in influent and effluent samples from all treatment plants (100%) throughout the year, and G. duodenalis always outnumbered Cryptosporidium spp. The mean concentration of G. duodenalis per liter of influent was significantly higher (P