The GW182 protein colocalizes with mRNA degradation associated proteins hDcp1 and hLSm4 in cytoplasmic GW bodies
The GW182 protein colocalizes with mRNA degradation associated proteins hDcp1 and hLSm4 in cytoplasmic GW bodies
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DOI：
10.1261/rna.5810203
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发表时间：
2003-10-01
期刊：
影响因子：
4.5
通讯作者：
Fritzler, MJ
中科院分区：
文献类型：
--
作者：
Eystathioy, T;Jakymiw, A;Fritzler, MJ
一种被称为GW小体（GWBs）的新型细胞质区室最初是利用针对一种被称为GW182的182 - kD蛋白的特异性抗体鉴定出来的。GW182的特征是具有多个甘氨酸（G）-色氨酸（W）重复序列以及一个RNA识别基序（RRM），它能结合一部分HeLa细胞信使RNA（mRNAs）。GW小体的功能尚不清楚；然而，最近的证据表明GW小体与包含hLSm蛋白和hDcp1（一种酵母脱帽酶亚基的人类同源物）的细胞质结构之间存在相似性。在这项研究中，我们利用针对hLSm4和hDcp1的抗体来表明，这两种mRNA降解途径的标志物与GW182共定位在相同的结构中。我们的研究证明GW182、hLSm4和hDcp1存在于相同的细胞质结构中，并表明GW182与hLSm4和hDcp1参与相同的mRNA加工途径。
A novel cytoplasmic compartment referred to as GW bodies (GWBs) was initially identified using antibodies specific to a 182-kD protein termed GW182. GW182 was characterized by multiple glycine(G)-tryptophan(W) repeats and an RNA recognition motif (RRM) that bound a subset of HeLa cell messenger RNAs (mRNAs). The function of GWBs was not known; however, more recent evidence suggested similarities between GWBs and cytoplasmic structures that contain hLSm proteins and hDcp1, the human homolog to a yeast decapping enzyme subunit. In this study, we used antibodies to hLSm4 and hDcp1 to show that both of these markers of an mRNA degradation pathway colocalize to the same structures as GW182. Our studies demonstrate that GW182, hLSm4, and hDcp1 are found in the same cytoplasmic structures and suggest that GW182 is involved in the same mRNA processing pathway as hLSm4 and hDcp1.