Antidiabetic effect of total saponins from Entada phaseoloides (L.) Merr. in type 2 diabetic rats
Antidiabetic effect of total saponins from Entada phaseoloides (L.) Merr. in type 2 diabetic rats
复制标题
菜豆总皂苷的抗糖尿病作用。
DOI：
10.1016/j.jep.2011.12.025
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发表时间：
2012-02-15
影响因子：
5.4
通讯作者：
Mei, Zhinan
中科院分区：
文献类型：
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作者：
Zheng, Tao;Shu, Guangwen;Mei, Zhinan
民族药理学相关性：Entada phaseoloides（L.）Merr.莲子草（Entada phaseoloides）是我国少数民族之一的傣族人长期使用的治疗糖尿病的有效药物。皂苷是该植物种子中含量丰富的次生代谢产物。本研究旨在探讨莲心总皂苷（total saponins from Entada phaseoloides，TSEP）对实验性2型糖尿病（type 2 diabetes mellitus，T2 DM）大鼠的治疗作用。TSEP按25、50、100 mg/kg剂量灌胃给药21 d。为了比较，使用标准抗糖尿病药物二甲双胍（200 mg/kg）作为阳性对照药物。结果：TSEP能显著降低T2 DM大鼠空腹血糖和血清胰岛素水平，减轻高血糖引起的氧化应激反应。此外，TSEP给药后可观察到显著的降血脂作用和组织脂肪变性的改善。进一步的研究通过检查血清白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）和C-反应蛋白（CRP）水平揭示了TSEP可能的抗炎作用。TSEP的作用呈剂量依赖性，与二甲双胍相当。结论：TSEP对2型糖尿病大鼠具有降血糖、降血脂作用，支持其抗糖尿病作用。这项工作也暗示了TSEP通过抑制慢性炎症反应发挥其治疗作用的可能性。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
Ethnopharmacological relevance: The seed of Entada phaseoloides (L.) Merr. (Entada phaseoloides) has been long used as an effective herb for the treatment of Diabetes mellitus by Dai people, one of the Chinese ethnic minorities. Saponin is an abundant type of secondary metabolic products in the seed of this plant. The aim of this study is to evaluate the potential therapeutic effects of total saponins from Entada phaseoloides (TSEP) in experimental type 2 Diabetes mellitus (T2DM) rats.Materials and methods: T2DM rats were induced by high-fat diet and low-dose streptozotocin (STZ). Then different oral doses of TSEP (25, 50 and 100 mg/kg) were administrated to T2DM rats for 21 days. For comparison, a standard antidiabetic drug, metformin (200 mg/kg), was used as a positive control drug. Then the relative biochemical analysis and histopathological examination were made to evaluate the antidiabetic effect of TSEP.Results: TSEP dramatically reduced fasted blood glucose and serum insulin levels and alleviates hyperglycemia associated oxidative stress in T2DM rats. Moreover, a significantly hypolipidemic effect and an improvement in tissue steatosis could be observed after TSEP administration. Further investigations revealed a possible anti-inflammation effect of TSEP by examining serum levels of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha) and C-reactive protein (CRP). The effects of TSEP exhibited a dose-dependent manner and were comparable to metformin.Conclusion: Our present study demonstrates both hypoglycemic and hypolipidemic activities of TSEP in T2DM rats, which support its antidiabetic property. This work also implies a possibility that TSEP exerts its therapeutic effect through repressing chronic inflammation responses. (C) 2011 Elsevier Ireland Ltd. All rights reserved.