Early endosomes, late endosomes, and lysosomes display distinct partitioning strategies of inheritance with similarities to Golgi-derived membranes
Early endosomes, late endosomes, and lysosomes display distinct partitioning strategies of inheritance with similarities to Golgi-derived membranes
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DOI：
10.1078/0171-9335-00232
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发表时间：
2002-03-01
影响因子：
6.6
通讯作者：
Sentry, JW
中科院分区：
文献类型：
--
作者：
Dunster, K;Toh, BH;Sentry, JW
内吞途径隔室的遗传模式很少报道，确切的机制尚未阐明。我们使用与早期内体（抗EEA 1）、晚期内体（抗LBPA和抗LAMP-1）、溶酶体（抗LAMP-1）和反式高尔基体网络（TGN）（抗GOLGA 4）反应的抗体来检查固定的人HEp-2细胞中这些区室的遗传。在进入M期之前，这些隔室在其细胞质分布中显示出核周偏置，其中局部积累区域与中心体并列。在有丝分裂过程中，这些隔间的位置进行了检查相对于彼此，染色体，中心体和微管网络。在M期，早期内体和TGN衍生的隔室共享重叠的亚细胞分布。这些车厢的一部分显示可辨别的集群周围分离和迁移的中心体在前期。在中期，这些隔间与有丝分裂纺锤体共定位，在中期板上不存在，并且不覆盖星形微管。在后期，这些隔间集中在缩短的动粒微管和中心体之间。此外，它们似乎分布在细胞体中伸长的极性微管上。从末期到胞质分裂，这些隔间集中在极纺锤体微管收缩残余的负端周围，并重新建立一个与中心体并列的突出存在。与此相反，几乎没有证据表明晚期内体和溶酶体的运动与迁移的中心体在前期，这些车厢被排除在有丝分裂纺锤体在中期。然而，到末期结束时，一部分晚期内体和溶酶体的亚细胞分布与早期内体的分布重叠。我们得出的结论是，部分内体隔室和高尔基体衍生的膜根据中心体和有丝分裂纺锤体进行有序分配。
The pattern of inheritance of compartments of the endocytic pathway has been rarely reported, and the precise mechanism(s) are yet to be elucidated. We used antibodies reactive to early endosomes (anti-EEA1), late endosomes (anti-LBPA and anti-LAMP-1), lysosomes (anti-LAMP-1) and trans-Golgi network (TGN) (anti-GOLGA4) to examine the inheritance of these compartments in fixed human HEp-2 cells. Prior to entering M phase, these compartments display a perinuclear bias in their cytoplasmic distribution with areas of local accumulation juxtaposed to the centrosome. The location of these compartments during mitosis was examined relative to each other, the chromosomes, centrosomes and the microtubule network. During M phase early endosomes and TGN-derived compartments share overlapping subcellular distributions. A portion of these compartments display discernible clustering around the separated and migrating centrosomes in prophase. At metaphase these compartments co-localise with the mitotic spindle, are absent at the metaphase plate and do not overlay the astral microtubules. At anaphase these compartments are concentrated between shortening kinetochore microtubules and centrosomes. In addition, they appear distributed over the elongating polar microtubules in the body of the cell. From telophase and into cytokinesis these compartments concentrate around the minus ends of the constricted remnants of polar spindle microtubules and re-establish a prominent presence juxtaposed to the centrosome. In contrast, there is little evidence of movement of late endosomes and lysosomes with migrating centrosomes in prophase, and these compartments are excluded from the mitotic spindle at metaphase. However, by the end of telophase, the subcellular distribution of a portion of late endosomes and lysosomes share overlapping distributions with that of early endosomes. We conclude a portion of endosomal compartments and Golgi-derived membranes undergo ordered partitioning based on the centrosome and mitotic spindle.