The impact of photodynamic therapy on the viability of Streptococcus mutans in a planktonic culture
The impact of photodynamic therapy on the viability of Streptococcus mutans in a planktonic culture
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DOI：
10.1089/pho.2007.2109
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发表时间：
2007-12-01
影响因子：
--
通讯作者：
Pacheco, M. T. T.
中科院分区：
文献类型：
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作者：
Bevilacqua, I. M.;Nicolau, R. A.;Pacheco, M. T. T.
目的：本研究探讨了甲苯胺蓝O（TBO）光动力疗法（PDT）和发光二极管（LED）对变形链球菌细胞活力的影响。背景资料：变形链球菌的生长是蛀牙发生的第一步。光和染料的使用以非侵入性的方式促进细胞死亡，减少治疗时间。方法：本实验所用LED的输出功率为116 mW，能量为21 J，光通量为2.18J/cm 2。制备样品并分成五组：（1）对照组（-）;（2）对照组（+）;（3）TBO;（4）LED;和（5）LED + TBO。结果：LED和TBO照射后，细菌杀灭率均为100%。通过扫描电镜和菌落计数对玻璃表面形成的生物膜进行分析。结论：光动力疗法能有效杀灭微生物，防止生物膜的形成。
Objective: This study investigated the effect of photodynamic therapy (PDT) with toluidine blue O (TBO) and a light-emitting diode (LED) on the viability of Streptococcus mutans cells in a planktonic culture. Background Data: Growth of Streptococcus mutans is the first step in the development of tooth decay. The use of light and dyes promotes cellular death in a noninvasive way, reducing treatment time. Methods: The LED used in this study had output power of 116 mW, its energy was 21 J, and the fluency was 2.18 J/cm(2). Samples were prepared and divided into five groups: (1) control group (-); (2) control group (+); (3) TBO; (4) LED; and (5) LED + TBO. Results: One hundred percent of the bacteria were killed following irradiation with LED and TBO. The biofilm that formed on the glass surfaces was analyzed by SEM and colony count. Conclusions: It was demonstrated that PDT was efficient at killing microorganisms and preventing the formation of biofilms.