Effects of pH on cell fusion induced by electric fields.
Effects of pH on cell fusion induced by electric fields.
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pH 对电场诱导的细胞融合的影响。
DOI：
10.1007/bf02797270
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Gao,PQ
中科院分区：
文献类型：
--
作者：
Chang,DC;Hunt,JR;Gao,PQ
电融合是近年来细胞生物学研究的一个重要领域。我们研究了细胞培养基的pH值对人红细胞电融合的影响。通过使用荧光显微镜观察相邻融合细胞之间亲脂染料的移动来监测细胞融合。首先通过介电泳使细胞紧密接触。然后通过三个高强度电场脉冲诱导融合。在施加脉冲后的几分钟内，观察到许多细胞融合在一起以形成不同长度的融合链。我们发现，细胞融合的最佳pH值约为pH 7.5。在此pH下，融合产率最高（范围为57 - 81%），融合链内的平均细胞数也最大。细胞融合对pH的依赖性在低pH下比在高pH下更敏感。当pH从7.5变化到6.0时，融合产量下降40%，但在pH 7.5和10.0之间，产量仅下降20%。我们怀疑观察到的pH效应可能是由细胞表面固定电荷的重新分布引起的，或表面蛋白的两亲性的变化。
Electrofusion has recently become an important area of cell biology research. We studied the effects of pH of the cell medium on the electrofusion of human red blood cells. Cell fusion was monitored by observing the movement of a lipophylic dye between neighboring fused cells using a fluorescence microscope. The cells were first brought into close contact by dielectrophoresis. Fusion was then induced by three pulses of high-intensity electric field. Within minutes following the pulse application, many cells were observed to fuse together to form fusion chains of different lengths. We found that the optimal pH for cell fusion is around pH 7.5. At this pH, the fusion yield was highest (ranging from 57 to 81%) and the average number of cells within a fusion chain was also the largest. The dependence of cell fusion on pH is more sensitive at low than at high pH. The fusion yield was decreased by 40% when the pH was changed from 7.5 to 6.0, but there was only a 20% decrease in yield between pH 7.5 and 10.0 We suspect that the observed pH effects may be caused by a redistribution of fixed charges at the cell surface, or changes in amphipathicity of the surface proteins.