Preparation of collagen porous scaffolds with controlled and sustained release of bioactive insulin
Preparation of collagen porous scaffolds with controlled and sustained release of bioactive insulin
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DOI：
10.1177/0883911514522724
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发表时间：
2014-02
影响因子：
1.7
通讯作者：
H. S. Nanda;N. Kawazoe;Qin Zhang;Shangwu Chen;Guoping Chen
中科院分区：
文献类型：
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作者：
H. S. Nanda;N. Kawazoe;Qin Zhang;Shangwu Chen;Guoping Chen
将生物活性生长因子从多孔支架中控制和局部释放，可以提供一种有效的策略来控制三维微环境中细胞过程的复杂和动态调节。本研究将胰岛素释放的聚乳酸-羟基乙酸共聚微球引入胶原多孔性支架中，制备了胶原-聚乳酸-羟基乙酸共聚微球杂化支架。用19.4±1.6和4.4±0.9µm两种不同大小的胰岛素包被的聚乳酸-乙醇酸共聚微球制备了杂化支架。支架具有可控的孔结构，聚乳酸-乙醇酸共聚微球均匀分布在支架的孔壁上。与游离微珠相比，支架的初始破裂时间更短，胰岛素释放更稳定。杂化支架中培养的人真皮成纤维细胞对胰岛素释放的生物活性有影响。与其他杂化组和对照组相比，19.4±1.6µm微珠制备的杂化支架具有更好的胰岛素线性释放和更强的细胞增殖促进作用。该杂化支架有望应用于皮肤组织工程。
Controlled and local release of bioactive growth factors from porous scaffolds can provide an efficient strategy to control the complex and dynamic regulation of cellular processes in a three-dimensional microenvironment. In this study, hybrid scaffolds of collagen and poly(lactic-co-glycolic acid) microbeads were prepared by introducing insulin-releasing poly(lactic-co-glycolic acid) microbeads into collagen porous scaffolds. Insulin-incorporated poly(lactic-co-glycolic acid) microbeads of two distinct sizes, 19.4 ± 1.6 and 4.4 ± 0.9 µm, were used to prepare the hybrid scaffolds. The scaffolds had controlled pore structure, and the poly(lactic-co-glycolic acid) microbeads were well distributed on the pore walls of the scaffolds. The scaffolds had a lower initial burst and a more stable insulin release than did the free microbeads. Culture of human dermal fibroblasts in the hybrid scaffolds were affected the bioactivity of released insulin. The hybrid scaffold prepared with 19.4 ± 1.6 µm microbeads had a more linear release of insulin and a higher promotion effect on cell proliferation than did the other hybrid group and control scaffolds. The hybrid scaffold should be useful for skin tissue engineering.