AU-rich RNA-binding induces changes in the quaternary structure of AUH
AU-rich RNA-binding induces changes in the quaternary structure of AUH
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DOI：
10.1002/prot.22246
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发表时间：
2009-05-01
影响因子：
2.9
通讯作者：
Yokoyama, Shigeyuki
中科院分区：
文献类型：
--
作者：
Kurimoto, Kazuki;Kuwasako, Kanako;Yokoyama, Shigeyuki
人Au RNA结合蛋白/烯酰辅酶A水合酶（AUH）是亮氨酸降解途径中的3-羟基-3-甲基戊烯二酰辅酶A水合酶。它还具有与AUUU重复序列的RNA结合活性，这不涉及已知的保守RNA结合结构域，并且似乎与酶活性无关。在这项研究中，我们进行了质谱分析，以阐明在RNA的存在和不存在的AUH的寡聚状态。在有短RNA（AUUU）或无RNA的情况下，AUH主要以三聚体形式存在于溶液中。另一方面，AUH三聚体在与含有24个重复的AUUU基序（AUUU）（24）A的长RNA的一个分子结合后二聚化。AUH与长RNA一起结晶。尽管RNA在晶格中是无序的，但AUH结构被确定为三聚体的不对称二聚体，在三聚体轴的排列中具有扭结，导致形成具有显著不同尺寸的两个裂缝。
The human AU RNA binding protein/enoyl-Coenzyme A hydratase (AUH) is a 3-hydroxy-3-methylglutaconyl-CoA dehydratase in the leucine degradation pathway. It also possesses an RNA-binding activity to AUUU repeats, which involves no known conserved RNA-binding domains and is seemingly unrelated to the enzymatic activity. In this study, we performed mass spectrometric analyses to elucidate the oligomeric states of AUH in the presence and absence of RNA. With a short RNA (AUUU) or without RNA, AUH mainly exists as a trimer in solution. On the other hand, the AUH trimer dimerizes upon binding to one molecule of a long RNA containing 24 repeats of the AUUU motif, (AUUU)(24)A. AUH was crystallized with the long RNA. Although the RNA was disordered in the crystalline lattice, the AUH structure was determined as an asymmetric dimer of trimers with a kink in the alignment of the trimer axes, resulting in the formation of two clefts with significantly different sizes.