Zebrafish Transgenic Constructs Label Specific Neurons in Xenopus laevis Spinal Cord and Identify Frog V0v Spinal Neurons
Zebrafish Transgenic Constructs Label Specific Neurons in Xenopus laevis Spinal Cord and Identify Frog V0v Spinal Neurons
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DOI：
10.1002/dneu.22490
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发表时间：
2017-09-01
影响因子：
3
通讯作者：
Lewis，Katharine E.
中科院分区：
文献类型：
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作者：
Juarez-Morales，Jose L.;Martinez-De Luna，Reyna I.;Lewis，Katharine E.
正常运作的脊髓对于身体和大脑之间的运动和交流至关重要，但我们对脊髓神经元回路如何建立和发挥作用的知识存在根本差距。为了了解调节该电路的规范和功能的遗传程序，我们需要将神经元分子表型与生理分析联系起来。使用爪蟾的研究增加了我们对脊髓神经元生理学和功能的了解，特别是在运动回路方面。然而，X.左旋四倍体基因组和长世代时间使得研究神经元如何被指定变得困难。 X 的不透明度。左胚胎也使得将神经元的功能类别与其表达的基因联系起来变得困难。我们在此证明，Tol2 转基因构建体使用斑马鱼增强子驱动特定斑马鱼脊髓神经元中的表达，标记了 X 中的等效神经元。 laevisand 认为，加入 aGal4:UASamplification 盒可以在 liveX 中观察细胞。莱维斯塔波尔斯。该技术应该能够实现distinctX的分子表型、形态和生理学。左旋脊髓神经元在体内一起检查。我们首次使用 anislet1 增强子来标记 Rohon-Beard 感觉神经元，并使用 evx 增强子来识别 V0v 神经元。莱维斯脊髓。我们的工作证明了斑马鱼和 X 脊髓回路的同源性。 laevis 表示，未来的工作可以结合它们的相对优势，以更全面地阐明脊椎动物脊髓神经元是如何被指定的，以及如何产生行为的功能。 © 2017 Wiley periodicals, Inc. 开发 Neurobiol 77: 1007–1020, 2017
A correctly functioning spinal cord is crucial for locomotion and communication between body and brain but there are fundamental gaps in our knowledge of how spinal neuronal circuitry is established and functions. To understand the genetic program that regulates specification and functions of this circuitry, we need to connect neuronal molecular phenotypes with physiological analyses. Studies usingXenopus laevistadpoles have increased our understanding of spinal cord neuronal physiology and function, particularly in locomotor circuitry. However, theX. laevistetraploid genome and long generation time make it difficult to investigate how neurons are specified. The opacity ofX. laevisembryos also makes it hard to connect functional classes of neurons and the genes that they express. We demonstrate here that Tol2 transgenic constructs using zebrafish enhancers that drive expression in specific zebrafish spinal neurons label equivalent neurons inX. laevisand that the incorporation of aGal4:UASamplification cassette enables cells to be observed in liveX. laevistadpoles. This technique should enable the molecular phenotypes, morphologies and physiologies of distinctX. laevisspinal neurons to be examined togetherin vivo. We have used anislet1enhancer to label Rohon‐Beard sensory neurons andevxenhancers to identify V0v neurons, for the first time, inX. laevisspinal cord. Our work demonstrates the homology of spinal cord circuitry in zebrafish andX. laevis, suggesting that future work could combine their relative strengths to elucidate a more complete picture of how vertebrate spinal cord neurons are specified, and function to generate behavior. © 2017 Wiley Periodicals, Inc. Develop Neurobiol 77: 1007–1020, 2017