FOLLICULAR-FLUID LIPOPROTEINS IN THE MARE - EVALUATION OF HDL TRANSFER FROM PLASMA TO FOLLICULAR-FLUID
FOLLICULAR-FLUID LIPOPROTEINS IN THE MARE - EVALUATION OF HDL TRANSFER FROM PLASMA TO FOLLICULAR-FLUID
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DOI：
10.1016/0005-2760(94)90125-2
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发表时间：
1994-01-03
期刊：
影响因子：
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通讯作者：
LEGOFF, D
中科院分区：
文献类型：
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作者：
LEGOFF, D
利用密度梯度超速离心法，我们分离了马血浆和卵泡液中的高密度脂蛋白。与血浆高密度脂蛋白相比，卵泡液中高密度脂蛋白的密度分布明显向最高密度方向移动。同样，大小分布分析显示，与血浆高密度脂蛋白(5.5至9.5 nm)相比，卵泡液高密度脂蛋白直径(4.2至9.2 nm)减小。根据离心管中苏丹黑染色带的分布，将高密度脂蛋白分离为三个亚组分。卵泡液中各亚组分的浓度明显较低，相对血浆当量百分比与相对分子质量成反比(高密度脂蛋白-1为23.8%，高密度脂蛋白-2为49.9%，高密度脂蛋白-3为63.7%)。在卵泡高密度脂蛋白-2和高密度脂蛋白-3亚组分中，胆固醇/磷脂摩尔比和酯化/游离胆固醇摩尔比明显增加。卵泡液中的载脂蛋白分布与血浆中的分布非常接近。在人和马的卵泡液中测得的LCAT活性比这两个物种的血浆中的LCAT活性弱(每毫升每小时酯化的游离胆固醇4.0n摩尔，每毫升每小时24n摩尔)。卵泡液高密度脂蛋白的理论浓度是在假设高密度脂蛋白颗粒只是一个过滤产物，没有可检测到的代谢修饰的情况下计算的。生化测量表明，最轻的颗粒(高密度脂蛋白-1)的数量比理论计算所建议的要少。因此，尽管卵泡液高密度脂蛋白似乎是血浆高密度脂蛋白的过滤产物，但它们经历了代谢转换，我们认为这可能与激素合成和反向胆固醇运输有关。
Using a density gradient ultracentrifugal procedure, we have separated equine plasma and follicular fluid high-density lipoproteins (HDL). The density distribution of the follicular fluid HDL was clearly displaced towards the highest densities in comparison with that of plasma HDL. Similarly, an analysis of size distributions showed a decrease in follicular fluid HDL diameters (4.2 to 9.2 nm) compared to plasma HDL (5.5 to 9.5 nm). HDL were isolated into three subfractions on the basis of the disposition of the Sudan Black stained bands in the centrifuge tubes. Concentrations of each subfraction were clearly lower in the follicular fluid, and the relative percentages with regard to the plasma equivalents were inversely proportional to the molecular weights (23.8% for HDL-1, 49.9% for HDL-2 and 63.7% for HDL-3). The cholesterol/phospholipid molar ratio and the esterified/free cholesterol molar ratio were clearly increased in the follicular HDL-2 and HDL-3 subfractions. The apolipoprotein distribution in follicular fluid HDL was very close to that in plasma HDL. LCAT activity measured in human as well as equine samples was weaker in follicular fluid compared to plasma in both species (4.0 nmol of free cholesterol esterified per h per ml vs. 24 nmol per h per ml). Theoretical concentrations of follicular fluid HDL were calculated assuming that the HDL particles would be merely a filtration product undergoing no detectable metabolic modifications. Biochemical measurements showed that the lightest particules (HDL-1) were less numerous than suggested by the theoretical calculation. Thus, although follicular fluid HDL appear to be a filtration product of plasma HDL, they undergo metabolic transformations that we suggest may be linked to hormonal synthesis and reverse cholesterol transport.