Inositol phospholipid turnover in PAF transmembrane signalling.
Inositol phospholipid turnover in PAF transmembrane signalling.
复制标题
PAF 跨膜信号传导中的肌醇磷脂周转。
作者：
Shukla,SD
在多种细胞和组织中，血小板激活因子(PAF)刺激磷脂酶C催化磷脂酰肌醇的分解。这导致第二信使--三磷酸肌醇和二甘油酯的产生。这一过程独立于细胞外钙离子而发生。许多PAF结构类似物、受体拮抗剂和药物已被用来从药理上探索磷脂酶C的激活。PAF对磷脂酶C的刺激在某些系统中对百日咳毒素敏感，而在另一些系统中则不敏感。鸟嘌呤核苷酸结合蛋白(S)和酪氨酸激酶(S)也参与了这一过程。这些进展在分子水平上为PAF受体的功能提供了新的见解，也为更好地理解细胞对PAF的反应提供了线索。
In a variety of cells and tissues, platelet activating factor (PAF) stimulates phospholipase C catalyzed breakdown of phosphoinositides. This results in the generation of the second messengers, inositol trisphosphate and diglyceride. This process occurs independently of extracellular Ca2+. A number of PAF structural analogues, receptor antagonists and drugs have been utilized to pharmacologically probe the activation of phospholipase C. PAF stimulation of the phosphoinositide turnover was shown to be sensitive to pertussis toxin in some systems, but not in others. The involvement of guanine nucleotide binding protein(s) and tyrosine kinase(s) in this process have also been postulated. These developments give new insights into PAF-receptor function at the molecular level, and also provide leads towards a better understanding of the cellular responses to PAF.