Whole cell assays in screening for biologically active substances
Whole cell assays in screening for biologically active substances
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DOI：
10.1016/0958-1669(93)90037-w
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发表时间：
1993-01-01
影响因子：
7.7
通讯作者：
Hertzberg, Robert P.
中科院分区：
文献类型：
--
作者：
Hertzberg, Robert P.
在过去的一年里，重组细胞检测出现了一些新的发展。人们开始使用基于机制的全细胞分析来鉴定先导化合物，以发现细胞过程的抑制因素，如反式激活和信号转导。与含有纯化大分子的亚细胞检测系统相比，这些检测方法为许多靶点提供了优势。
This past year has seen a number of new developments in recombinant cell based assays. Lead compounds are beginning to be identified using mechanism-based whole cell assays to discover inhibitors of cellular processes, such as transactivation and signaling. These assays offer advantages for many targets over subcellular assay systems with purified macromolecules.