Modeling the elimination of mature biofilms formed by Staphylococcus aureus and Salmonella spp. Using combined ultrasound and disinfectants
Modeling the elimination of mature biofilms formed by Staphylococcus aureus and Salmonella spp. Using combined ultrasound and disinfectants
复制标题
模拟金黄色葡萄球菌和沙门氏菌形成的成熟生物膜的消除。
DOI：
10.1016/j.ultsonch.2020.105269
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发表时间：
2020-12-01
影响因子：
8.4
通讯作者：
Wang, Huhu
中科院分区：
文献类型：
--
作者：
Shao, Liangting;Dong, Yang;Wang, Huhu
食源性病原体在食品加工表面形成的生物膜导致了许多疾病的爆发和食品召回。我们评估了以下消除金黄色葡萄球菌和沙门氏菌形成的成熟生物膜的策略。不锈钢表面：酸性电解水（AEW）、臭氧水（OW）或单独的超声波（40 kHz），以及超声波和消毒剂的组合。使用威布尔和双相模型确定了组合的消除动力学。单独使用AEW处理可使生物膜细胞数量减少约3.0 log cfu/cm（2），而单独使用OW或超声处理生物膜，即使处理20 min，也观察到不到0.8 log cfu/cm（2）的细胞减少。AEW和超声的组合对生物膜减少产生明显的协同效应，沙门氏菌减少约4.8 log cfu/cm（2）。生物膜.有趣的是，对于由两种病原体形成的成熟生物膜的消除过程，双相模型比Weibull模型更好地拟合，并经受超声和AEW的组合，如统计参数RMSE和AIC的较小值所确定的，尽管两种模型都可以评估动态过程。我们的研究结果表明，超声和空气预警的组合可以有效地减少由病原体在食品接触表面形成的生物膜，并且双相模型可以预测生物膜暴露于这种干预方法后的残留细胞数量。
Biofilm formation by foodborne pathogens on food processing surfaces has contributed to numerous disease outbreaks and food recalls. We evaluated the following strategies for elimination of mature biofilm formed by Staphylococcus aureus and Salmonella spp. on stainless steel surfaces: acidic electrolyzed water (AEW), ozone water (OW), or ultrasound (40 kHz) alone, and combinations of ultrasound and disinfectants. The dynamics of elimination by combinations were determined using the Weibull and biphasic models. Treatment with AEW alone reduced the number of biofilm cells by approximately 3.0 log cfu/cm(2), whereas less than 0.8 log cfu/cm(2) of cells reduction was observed in biofilm exposed to OW or ultrasound alone, even with treatment for 20 min. The combination of AEW and ultrasound produced an obvious synergistic effect on biofilm reduction, achieving approximately 4.8 log cfu/cm(2) reduction in Salmonella spp. biofilm. Interestingly, the biphasic model was a better fit than the Weibull model for the elimination process of mature biofilm formed by both pathogens and subjected to a combination of ultrasound and AEW, as determined by smaller values of the statistical parameters RMSE and AIC, although both models could evaluate the dynamic processes. Our findings indicated that a combination of ultrasound and AEW could effectively reduce the biofilm formed by pathogens on food contact surfaces, and that the biphasic model could predict the number of residual cells after biofilm exposure to this intervention approach.