THE ORAL GRAM-NEGATIVE ANAEROBIC MICROFLORA IN YOUNG-CHILDREN - LONGITUDINAL CHANGES FROM EDENTULOUS TO DENTATE MOUTH
THE ORAL GRAM-NEGATIVE ANAEROBIC MICROFLORA IN YOUNG-CHILDREN - LONGITUDINAL CHANGES FROM EDENTULOUS TO DENTATE MOUTH
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DOI：
10.1111/j.1399-302x.1994.tb00049.x
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发表时间：
1994-06-01
影响因子：
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通讯作者：
JOUSIMIESSOMER, H
中科院分区：
文献类型：
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作者：
KONONEN, E;ASIKAINEN, S;JOUSIMIESSOMER, H
乳牙的萌出通过为细菌定植提供合适的小生境而对口腔环境具有很大的影响。本文对21名平均年龄32个月的乳牙期儿童口腔革兰氏阴性厌氧菌群进行了调查。将样本的细菌结果与在无牙婴儿期（平均年龄3个月）收集的相同儿童的细菌结果进行比较。在乳牙期，从每名儿童收集2份样本：从2颗牙齿的龈缘收集牙线样本，并将刺激唾液与粘膜拭子样本混合。使用非选择性和选择性培养基对两份样本进行需氧和厌氧培养。产黑素普雷沃氏菌，无色素普雷沃氏菌属，具核梭杆菌群和二氧化碳噬纤维菌属。而在无牙颌中分别为76%、62%、67%和19%。结果表明，在不同的监测点，雷氏普雷沃菌的检出率从14%上升到90%，中间普雷沃菌的检出率从10%上升到67%;弯曲杆菌（Wolinella）spp.）Eikenella corrodens为5 ~ 57%。只有纤细拟杆菌和韦荣氏球菌的出现。保持在同一水平。未从无牙口腔中分离出的菌种，如生牙普雷沃氏菌、梭杆菌属。除具核克雷伯氏菌群和月形单胞菌属（Selenomonaspp.）现在分别有71%、71%和43%的儿童被发现。通过使用核糖分型确定口腔中定殖产黑色素假单胞菌菌株的稳定性;分析来自9名儿童的无牙婴儿期的每个儿童1-2个分离株和来自其乳牙期的每个儿童3-15个分离株。黑色素原卟啉单胞菌群体被证明是个体内非常异质的，但有一个孩子在婴儿和儿童时期具有相同的核糖体型。虽然在无牙口腔中稳定的定植似乎已经是可能的，但定植在口腔表面的新物种或菌株在乳牙列幼儿中产生了多种形式的革兰氏阴性厌氧微生物菌群。
Eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. The composition of oral gram-negative anaerobic microflora was investigated in 21 young children (mean age 32 months) with primary dentition. The bacterial findings of samples were compared with those of the same children collected at their edentulous infant period (mean age 3 months). During the primary dentition period, 2 samples were collected from each child: a sample with dental floss from gingival margin of 2 teeth and stimulated saliva pooled with a mucosal swab sample. Both samples were cultured aerobically and anaerobically using nonselective and selective media. Prevotella melaninogenica, nonpigmented Prevotella spp., Fusobacterium nucleatum group and Capnocytophaga spp. were found in all children at the older age, whereas they occurred in edentulous mouth in 76%, 62%, 67% and 19%, respectively. The occurrence of Prevotella loescheii increased from 14% to 90%, Prevotella intermedia from 10% to 67%, Leptotrichia spp. from 24 to 71%, Campylobacter (Wolinella) spp. from 5 to 43% and Eikenella corrodens from 5 to 57%. Only the occurrence of Bacteroides gracilis and Veillonella spp. remained at about the same level. Species not isolated from the edentulous mouth, such as Prevotella denticola, Fusobacterium spp. other than the K nucleatum group and Selenomonas spp. were now detected in 71%, 71% and 43% of the children. The stability of the colonizing P. melaninogenica strain(s) in the oral cavity was determined by using ribotyping; 1-2 isolates per child from the edentulous infant period of 9 children and 3-15 isolates per child from their primary dentition period were analyzed. The P. melaninogenica population proved to be intraindividually very heterogeneous, but one child had the same ribotype both in the infant and child period. Although a stable colonization seems to be possible already in the edentulous mouth, new species or strains colonizing oral surfaces create a multiform gram-negative anaerobic microflora in young children with primary dentition.