Rejoining of DNA by the RAG1 and RAG2 proteins
Rejoining of DNA by the RAG1 and RAG2 proteins
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DOI：
10.1126/science.280.5361.301
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发表时间：
1998-04-10
期刊：
影响因子：
56.9
通讯作者：
van Gent, DC
中科院分区：
文献类型：
--
作者：
Melek, M;Gellert, M;van Gent, DC
从不同的基因片段组装免疫球蛋白和T细胞受体基因[V(D)J重组]始于RAG1和RAG2蛋白作用于一对重组信号序列(RSS)的DNA双链断裂。这里，RAG蛋白被证明通过将RSS连接到断裂的编码序列末端来逆转切割反应。即使在DNA双链断裂修复的正常途径不活跃的情况下，这些“杂交关节”也在淋巴样细胞中被发现，现在可以用RAG蛋白的这种活性来解释。
Assembly of immunoglobulin and T cell receptor genes from separate gene segments [V(D)J recombination] begins with DNA double-strand breakage by the RAG1 and RAG2 proteins, acting at a pair of recombination signal sequences (RSSs). Here, the RAG proteins are shown to reverse the cleavage reaction by joining an RSS to a broken coding sequence end. These "hybrid joints" have also been found in lymphoid cells, even when the normal pathway of DNA double-strand break repair is inactive, and can now be explained by this activity of the RAG proteins.