Stimulation of osteogenic differentiation and inhibition of adipogenic differentiation in bone marrow stromal cells by alendronate via ERK and JNK activation
Stimulation of osteogenic differentiation and inhibition of adipogenic differentiation in bone marrow stromal cells by alendronate via ERK and JNK activation
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阿仑膦酸钠通过 ERK 和 JNK 激活刺激骨髓基质细胞成骨分化并抑制成脂分化
DOI：
10.1016/j.bone.2008.03.008
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发表时间：
2008-07-01
期刊：
影响因子：
4.1
通讯作者：
Dai, Kerong
中科院分区：
文献类型：
--
作者：
Fu, Lingjie;Tang, Tingting;Dai, Kerong
为了阐明双膦酸盐对骨代谢影响的机制，我们研究了阿仑膦酸钠，一种广泛使用的双膦酸盐，对来自去卵巢SD大鼠的骨髓基质细胞（BMSCs）成骨和成脂分化的影响。阿仑膦酸钠处理不仅增加了成骨诱导后骨形态发生蛋白-2、runt相关转录因子2、骨桥蛋白、骨唾液蛋白和碱性磷酸酶活性的mRNA水平，而且降低了成脂诱导后过氧化物酶体增殖物激活受体γ 2的mRNA水平和油红O染色显示的总液滴数。阿仑膦酸钠对成骨成脂能力的影响呈剂量依赖性，治疗组与未治疗组之间成骨成脂能力差异有统计学意义（p
To elucidate the mechanism of the effect of bisphosphonates on bone metabolism, we investigated the effect of alendronate, a widely used bisphosphonate, on osteogenic and adipogenic differentiation in bone marrow stromal cells (BMSCs) derived from ovariectomized SD rats. Alendronate treatment not only increased the mRNA level of bone morphogenetic protein-2, runt-related transcription factor 2, osteopontin, bone sialoprotein, and alkaline phosphatase activity after osteogenic induction, but also decreased the mRNA level of peroxisome proliferator activated receptor gamma 2 and total droplet number indicated by Oil Red O staining after adipogenic induction. The effect of alendronate treatment was dose-dependent, and the difference of the osteogenic or the adipogenic potential between the treated group and the non-treated group was statistically significant (p