An Experimental Study on Dynamic Morphological Changes and Expression Pattern of GFAP and Synapsin I in the Hippocampus of MTLE Models for Immature Rats
An Experimental Study on Dynamic Morphological Changes and Expression Pattern of GFAP and Synapsin I in the Hippocampus of MTLE Models for Immature Rats
复制标题
未成熟大鼠MTLE模型海马GFAP和突触蛋白I动态形态变化及表达模式的实验研究
DOI：
10.3109/00207454.2011.598979
复制
发表时间：
2011-10-01
影响因子：
2.2
通讯作者：
Yin, Fei
中科院分区：
文献类型：
--
作者：
Damaye, Camara Aboubacar;Wu, Liwen;Yin, Fei
摘要目的：建立类似人类内侧颞叶癫痫(MTLE)的动物模型，观察幼年大鼠海马区苔藓纤维发芽(MFS)和神经元丢失的动态变化，探讨幼鼠海马区胶质纤维酸性蛋白(GFAP)和突触素I(Synapsin I)的表达变化。方法：采用锂-匹罗卡品诱导幼年大鼠MTLE模型。对存活的动物进行连续监测8周。尼氏染色观察神经元丢失情况，TIMM染色评价MFS。用免疫组织化学方法检测GFAP和突触素I的表达。结果：94.1%的大鼠成功诱发癫痫持续状态，死亡率为68.8%，75%的存活大鼠出现类似人类MTLE特征的自发性反复发作。胶质纤维酸性蛋白和突触素I的表达水平随MTLE发育的不同阶段而波动。结论：利用幼年大鼠建立了人MTLE动物模型。GFAP和突触素I的表达参与了MTLE的发生发展。神经元丢失和苔藓纤维发芽可能在癫痫的发生中起一定作用。
ABSTRACT Objective: To establish an animal model resembling human mesial temporal lobe epilepsy (MTLE); observe the dynamic changes of mossy fiber sprouting (MFS) and neuron loss in the hippocampus; and investigate the expression changes of Glial fibrillary acidic protein (GFAP) and Synapsin I in the hippocampus in immature rats. Methods: MTLE models of immature rats were induced by lithium–pilocarpine. The surviving animals were continually monitored for 8 weeks. Nissl staining was used to observe the neuron loss and Timm staining was performed to evaluate MFS. Western blot (WB) and immunohistochemical methods were performed to detect the expression of GFAP and Synapsin I. Results: Status epilepticus (SE) was successfully induced in 94.1% of the rats with a high mortality of 68.8%; 75% of the survived rats were observed for spontaneous recurrent seizures (SRS) which resembles the features of human MTLE. Expression levels of glial fibrillary acidic protein and Synapsin I fluctuated in correspondence with the different stages of MTLE development. Conclusion: We established an animal model depicting the human MTLE by using immature rats. GFAP and Synapsin I expressions are involved in MTLE development. Neuron loss and mossy fiber sprouting may have a role in epileptogenesis.