An investigation of human brain tumour lipids by high-resolution magic angle spinning 1H MRS and histological analysis
An investigation of human brain tumour lipids by high-resolution magic angle spinning 1H MRS and histological analysis
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DOI：
10.1002/nbm.1239
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发表时间：
2008-08-01
影响因子：
2.9
通讯作者：
Howe, Franklyn A.
中科院分区：
文献类型：
--
作者：
Opstad, Kirstie S.;Bel, B. Anthony;Howe, Franklyn A.
通过H-1 MRS在体内检测到的NMR可见脂质信号与肿瘤侵袭相关，并被认为是由细胞质脂滴引起的。对人脑肿瘤活检标本进行高分辨率魔角旋转（HRMAS）H-1 MRS和尼罗红染色，以研究NMR可见脂质信号与不同级别胶质瘤中活细胞和坏死水平的关系。使用HRMAS H-1 MRS从24个成人星形细胞瘤活检样本（II级8个、III级2个和IV级14个）中获得预饱和光谱，并使用LC模型进行定量以确定脂质浓度。然后将每个活检样本重新冷冻，低温恒温器切片，并用尼罗红染色，以确定脂滴数量和液滴大小分布，并用苏木精和伊红染色，以确定细胞密度和坏死百分比。尼罗红染色液滴的数量与H-1 MRS测得的1.3ppm脂质质子浓度之间存在很强的相关性（R = 0.92，P < 0.0001）。液滴直径范围为1 - 10 μ m，大小分布与肿瘤分级无关。在非坏死活检样本中，脂滴数量与细胞密度相关，而在坏死样本中，有更多的液滴与坏死百分比呈正相关。1H MRS脂质信号与尼罗红染色液滴数量之间的相关性以及非坏死活检标本中脂滴的存在提供了良好的证据，证明体内NMR可见脂质信号起源于细胞质，并且脂滴的形成先于坏死。版权所有（C）2008年约翰威利父子。公司
NMR-visible lipid signals detected in vivo by H-1 MRS are associated with tumour aggression and believed to arise from cytoplasmic lipid droplets. High-resolution magic angle spinning (HRMAS) H-1 MRS and Nile Red staining were performed on human brain tumour biopsy specimens to investigate how NMR-visible lipid signals relate to viable cells and levels of necrosis across different grades of glioma. Presaturation spectra were acquired from 24 adult human astrocytoma biopsy samples of grades II (8) III (2) and IV (14) using HRMAS H-1 MRS and quantified using LCModel to determine lipid concentrations. Each biopsy sample was then refrozen, cryostat sectioned, and stained with Nile Red, to determine the number of lipid droplets and droplet size distribution, and with Haematoxylin and Eosin, to determine cell density and percentage necrosis. A strong correlation (R = 0.92, P < 0.0001) was found between the number of Nile Red-stained droplets and the similar to 1.3 ppm lipid proton concentration by H-1 MRS. Droplet sizes ranged from 1 to 10 mu m in diameter, and the size distribution was constant independent of tumour grade. In the non-necrotic biopsy samples, the number of lipid droplets Correlated with cell density, whereas in the necrotic samples, there were greater numbers of droplets that showed a positive correlation with percentage necrosis. The correlation between 1H MRS lipid signals and number of Nile Red-stained droplets, and the presence of lipid droplets in the non-necrotic biopsy specimens provide good evidence that the in vivo NMR-visible lipid signals are cytoplasmic in origin and that formation of lipid droplets precedes necrosis. Copyright (C) 2008 John Wiley & Sons. Ltd.