Development of Molecular Markers Based on the L1 Retrotransposon Insertion Polymorphisms in Pigs (Sus scrofa) and Their Association with Economic Traits
Development of Molecular Markers Based on the L1 Retrotransposon Insertion Polymorphisms in Pigs (Sus scrofa) and Their Association with Economic Traits
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DOI：
10.1134/s1022795420020131
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发表时间：
2020-02
影响因子：
0.6
通讯作者：
Wei Wang;Cai Chen;X. Wang;Li Zhang;D. Shen;Saisai Wang;B. Gao;Jiude Mao;Chengyi Song
中科院分区：
文献类型：
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作者：
Wei Wang;Cai Chen;X. Wang;Li Zhang;D. Shen;Saisai Wang;B. Gao;Jiude Mao;Chengyi Song
基于反转录转座子插入多态性（RTIPs）的分子标记在动物遗传育种研究中具有巨大的潜力。本研究利用微卫星引物结合L1特异引物，开发了L1 RTIP标记，并在4个中国猪种和3个进口家猪品种中进行了进一步的评价。共获得8个L1 RTIP标记，在不同品种间分布不均匀，其中苏江猪的多态性最高，有7个多态位点，其次是江曲海猪、梅山猪和杜洛克猪，分别有6个、4个和4个多态位点。长白猪和约克郡猪均表现出3个多态性位点，而中国近交系巴马猪的多态性水平最低，只有1个多态性位点。在462头约克郡猪中，3个多态标记（L1-31-2、L1-31-5和L1-31-11）与经济性状密切相关，L1-31-5+基因型母猪的活产仔数（11.44 ± 2.80）显著高于L1-31-5-基因型猪（9.96 ± 2.89）（P 0.05）。L1-31-11-基因型猪在100 kg体重时的日龄（160.20 ± 10.26天）显著低于L1-31-11+对照组（162.47 ± 9.71天）（P 0.05）。综上所述，本研究证明了利用L1 RTIP标记在猪中的应用是可行的，与非选择性常规育种相比，这些标记在猪的遗传育种中具有更大的应用潜力。
Molecular markers based on retrotransposon insertion polymorphisms (RTIPs) have great potential in studies of animal genetics and breeding. In this study, L1 RTIP markers were developed and evaluated by using microsatellite primers combined with L1 specific primers, and were further evaluated in four Chinese and three imported domestic pig breeds. Eight L1RTIP markers were obtained, and uneven distribution was observed among these breeds, with the Sujiang breed exhibiting the highest level of polymorphism with seven polymorphic sites, followed by Jiangquhai, Meishan, and Duroc with six, four, and four polymorphic sites, respectively. Both Landrace and Yorkshire pigs exhibited three polymorphic sites, while Chinese inbred Bama pig showed the lowest level of polymorphism with only one polymorphic site. Three polymorphic makers (L1-31-2, L1-31-5, and L1-31-11) were strongly associated with the economic traits in 462 Yorkshire pigs, with the L1-31-5+ genotype females had a significantly greater alive litter size (11.44 ± 2.80) compared with pigs with the L1-31-5– genotype (9.96 ± 2.89) (P < 0.05). L1-31-11– genotype pigs had significantly younger age at 100 kg of body weight (160.20 ± 10.26 days) than the L1-31-11+counter parts (162.47 ± 9.71 days) (P < 0.05). In summary, we demonstrated that it is feasible to develop markers based on L1 RTIP sin the pig, and these markers may have greater potential in the application of pig genetics and breeding compared to non-selective conventional breeding.