Pentatricopeptide repeat protein MID1 modulates nad2 intron 1 splicing and Arabidopsis development
Pentatricopeptide repeat protein MID1 modulates nad2 intron 1 splicing and Arabidopsis development
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DOI：
10.1038/s41598-020-58495-5
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发表时间：
2020
影响因子：
4.6
通讯作者：
Wei-Cai Yang
中科院分区：
文献类型：
--
作者：
Peng Zhao;Fang Wang;Na Li;Dong-Qiao Shi;Wei-Cai Yang
As one of the best-studied RNA binding proteins in plant, pentatricopeptide repeats (PPRs) protein are.mainly targeted to mitochondria and/or chloroplasts for RNA processing to regulate the biogenesis and.function of the organelles, but its molecular mechanism and role in development remain to be further.revealed. Here, we identified a mitochondria-localized P-type small PPR protein, MITOCHONDRIONMEDIATED.GROWTH DEFECT 1 (MID1) that is crucial for Arabidopsis development. Mutation in MID1.causes retarded embryo development and stunted plant growth with defects in cell expansion and.proliferation. Molecular experiments showed that MID1 is required for the splicing of the nad2 intron 1.in mitochondria. Consistently, mid1 plants display significant reduction in the abundance and activity of.mitochondrial respiration complex I, accompanied by abnormal mitochondrial morphology and energy.metabolism. Furthermore, MID1 is associated with other trans-factors involved in NICOTINAMIDE.ADENINE DINUCLEOTIDE HYDROGEN (NADH) DEHYDROGENASE SUBUNIT 2 (nad2) intron 1 splicing,.and interacts directly with itself and MITOCHONDRIAL STABILITY FACTOR 1 (MTSF1). This suggests.that MID1 most likely functions as a dimer for nad2 intron 1 splicing. Together, we characterized a novel.PPR protein MID1 for nad2 intron 1 splicing.
