Molecular characterization and embryonic expression of the family of N-methyl-D-aspartate receptor subunit genes in the zebrafish
Molecular characterization and embryonic expression of the family of N-methyl-D-aspartate receptor subunit genes in the zebrafish
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DOI：
10.1002/dvdy.20532
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发表时间：
2005-11-01
影响因子：
2.5
通讯作者：
Voigt, MM
中科院分区：
文献类型：
--
作者：
Cox, JA;Kucenas, S;Voigt, MM
本研究克隆了斑马鱼的10个N-甲基-D-天冬氨酸（NMDA）受体亚基。这些亚基分为五个亚型，每个亚型包含两个旁系同源基因。因此，我们报告了两个NMDAR 1基因（NR1.1和NR1.2）和八个NMDAR 2基因，命名为NR2A.1和NR2A.2，NR2B.1和NR2B.2，NR2C.1和NR2C.2，以及NR2D.1和NR2D.2。NR 1旁系同源物的预测序列与人蛋白质显示90%的同一性。NR 2亚基显示较少的同一性，在N-和C-末端最不同。NR 1的。两种基因都在胚胎中表达，尽管方式不同。NR1.1在受精后24小时（hpf）在大脑、视网膜和脊髓中发现。NR1.2在48 hpf时在脑中表达，但在脊髓中不表达。NR 2发育基因表达变化：NR 2A的两个旁系同源物在视网膜中48 hpf表达，NR 2B的仅一个旁系同源物在心脏中48 hpf以低水平表达。NR 2C在胚胎中均不表达。NR 2D的两种旁系同源物都有表达：2D.1在24 hpf时存在于前脑、视网膜和脊髓中，而2D.2仅存在于视网膜中。我们的研究结果表明，斑马鱼可以作为一个有用的模型系统，研究NMDA受体在神经系统发育中的作用。
We present the cloning of 10 N-methyl-D-aspartate (NMDA) receptor subunits from the zebrafish. These subunits fall into five subtypes, each containing two paralogous genes. Thus, we report two NMDAR1 genes (NR1.1 and NR1.2), and eight NMDAR2 genes, designated NR2A.1 and NR2A.2, NR2B.1 and NR2B.2, NR2C.1 and NR2C.2, and NR2D.1 and NR2D.2. The predicted sequences of the NR1 paralogs display 90% identity to the human protein. The NR2 subunits show less identity, differing most at the N- and C-termini. The NR1. genes are both expressed embryonically, although in a nonidentical manner. NR1.1 is found in brain, retina, and spinal cord at 24 hours postfertilization (hpf). NR1.2 is expressed in the brain at 48 hpf but not in the spinal cord. NR2 developmental gene expression varies: both paralogs of the NR2A are expressed at 48 hpf in the retina, only one paralog of the NR2B is expressed at low levels in the heart at 48 hpf. Neither of the NR2C is expressed embryonically. Both paralogs of the NR2D are expressed: 2D.1 is in the forebrain, retina, and spinal cord at 24 hpf, whereas the 2D.2 is only found in the retina. Our findings demonstrate that the zebrafish can serve as a useful model system for investigating the role of NMDA receptors in the development of the nervous system.