Dengue virus infection among children with undifferentiated fever in Karachi
Dengue virus infection among children with undifferentiated fever in Karachi
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DOI：
10.1007/bf02731055
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发表时间：
1998-09-01
影响因子：
4.3
通讯作者：
Takasu, T.
中科院分区：
文献类型：
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作者：
Akram, Dure Samin;Igarashi, Akira;Takasu, T.
1994年6 ~ 9月在巴基斯坦卡拉奇共采集了122例儿童血清，其中117例为未分化热，5例为登革出血热（DHF）。通过IgM捕获ELISA使用登革热1型（D1）、登革热2型（D2）、西尼罗河（WN）和日本脑炎（JE）病毒抗原检测血清。在92份未分化热患者单份血清中，D1抗原检测IgM抗体5例，D2抗原检测IgM抗体8例，WN抗原检测IgM抗体5例。25对未分化热患者血清中D_1抗原阳性3例，D_2抗原阳性6例，WN抗原阳性1例。5例DHF中4例同时具有抗D_1和抗D_2IgM抗体。仅1例DHF患者抗WN IgM抗体阳性。在所有受试血清标本中均未检出抗乙脑IgM抗体。未分化热患者的临床表现一般无特异性，但有抗登革IgM抗体者贫血、肝、脾肿大的比例高于无抗体者。在具有抗登革IgM抗体的组中，仅具有抗D2但不具有抗D1 IgM抗体的组显示出较高的咳嗽、水肿和脾肿大百分比。结果表明，巴基斯坦卡拉奇高达26%的未分化发热病例是由登革热病毒感染引起的。
Sera were collected from a total of 122 children comprising 117 cases with undifferentiated fever and 5 cases with dengue hemorrhagic fever (DHF), during June to September 1994 in Karachi, Pakistan. Sera were tested by the IgM-capture ELISA using dengue type 1 (D1), dengue type 2 (D2), West Nile (WN), and Japanese encephalitis (JE) viral antigens. Among 92 single sera from undifferentiated fever cases, IgM antibodies were detected in 5 cases by D1, 8 cases by D2, and 5 cases by WN antigens, respectively. Corresponding number of positive's among 25 paired sera from undifferentiated fever cases were 3 by D1, 6 by D2, and 1 by WN antigen. Four out of 5 DHF cases possessed arti-D1 as well as anti-D2 IgM antibodies. Only a single DHF case was positive for anti-WN IgM antibodies. Anti-JE IgM antibodies were not detected in any of the tested serum specimens. Clinical manifestations of undifferentiated fever patients were generally non specific, but the percentage of children with anemia, hepatomegaly and splenomegaly was higher in, patients possessing anti-dengue IgM antibodies than those without. Among the groups with anti-dengue IgM antibodies, those possessing only anti-D2 but not anti-D1 IgM antibodies showed higher percentages with cough, edema, and splenomegaly. The results indicated that up to 26% of the undifferentiated fever cases were caused by dengue virus infection in Karachi, Pakistan.