Intestinal IgA as a modulator of the gut microbiota
Intestinal IgA as a modulator of the gut microbiota
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DOI：
10.1080/19490976.2017.1310357
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发表时间：
2017-01-01
期刊：
影响因子：
12.2
通讯作者：
Shinkura, Reiko
中科院分区：
文献类型：
--
作者：
Okai, Shinsaku;Usui, Fumihito;Shinkura, Reiko
越来越多的证据表明，肠道菌群失调在包括炎症性肠病（IBD）以及肠外疾病在内的肠道疾病的发病机制中起作用。作为肠道微生物群的调节剂，我们分离出一种小鼠单克隆伊加抗体（克隆W27），其对多种肠道细菌具有高亲和力，但对有益细菌如干酪乳杆菌（Lactobacillus casei）没有亲和力。通过特异性识别丝氨酸羟甲基转移酶（SHMT）（一种细菌代谢酶）中的表位，W27伊加选择性抑制结合细菌（包括大肠杆菌（E. coli））的体外生长。coli），而对未结合的有益细菌如L. casei。通过调节体内肠道微生物群，口服施用W27伊加有效地预防了几种小鼠模型中结肠炎的发展。在这里，我们讨论了肠道伊加如何通过识别SHMT来调节肠道微生物群。
Accumulating evidence suggests that dysbiosis plays a role in the pathogenesis of intestinal diseases including inflammatory bowel disease (IBD) as well as extra-intestinal disorders. As a modulator of the intestinal microbiota, we isolated a mouse monoclonal IgA antibody (clone W27) with high affinities for multiple commensal bacteria, but not for beneficial bacteria such as Lactobacillus casei (L casei). Via specific recognition of an epitope in serine hydroxymethyltransferase (SHMT), a bacterial metabolic enzyme, W27 IgA selectively inhibited the in vitro growth of bound bacteria, including Escherichia coli (E. coli), while having no effect on unbound beneficial bacteria such as L. casei. By modulating the gut microbiota in vivo, oral administration of W27 IgA effectively prevented development of colitis in several mouse models. Here we discuss how intestinal IgA modulates the gut microbiota through recognition of SHMT.