Metal Organic Polyhedra: A Click-and-Clack Approach Toward Targeted Delivery.
Metal Organic Polyhedra: A Click-and-Clack Approach Toward Targeted Delivery.
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DOI：
10.1002/hlca.201800057
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发表时间：
2018-06
影响因子：
1.8
通讯作者：
S. Samanta;Damien Moncelet;B. Vinciguerra;V. Briken;L. Isaacs
中科院分区：
文献类型：
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作者：
S. Samanta;Damien Moncelet;B. Vinciguerra;V. Briken;L. Isaacs
配体1和2、PXDA(3)和Pd(NO3)2的混合自组装得到了金属有机多面体(MOP 1-MOP 3)，其中含有24个共价连接的CB[7]和环辛炔部分。共价菌株对MOP 3进行组装后修饰(PAM)，促进了炔叠氮点击反应，使MOP 4 R具有共价连接官能团(聚乙二醇、磺酸盐、生物素、c-RGD、荧光素和花青素)。通过CB[7]客体介导的MOP 4 R与Ad-FITC的非共价PAM反应，得到了MOP 5 RGD Ad-FITC和MOP 5 Biotin 0020Ad-FITC。流式细胞术分析MOP 5 RGD Ad-FITC对U87细胞的摄取显示，与缺乏c-RGD配体的对照MOP相比，MOP 5 RGD Ad-FITC的摄取增加。这些结果表明，可正交功能化(共价和非共价)MOPS在靶向给药和成像应用中具有广泛的适用性。
Mixed self-assembly of ligands 1 and 2, PXDA (3), and Pd(NO3)2 afforded metal organic polyhedra (MOP 1 - MOP 3) which bear 24 covalently attached CB[7] and cyclooctyne moieties. Post assembly modification (PAM) of MOP 3 by covalent strain promoted alkyne azide click reaction provided MOP 4 R bearing covalently attached functionality (PEG, sulfonate, biotin, c-RGD, fluorescein and cyanine). Orthogonal CB[7] guest mediated non-covalent PAM of MOP 4 R with Ad-FITC afforded MOP 5 RGD Ad-FITC and MOP 5 biotin 0020Ad-FITC. Flow cytometry analysis of the uptake of MOP 5 RGD Ad-FITC toward U87 cells demonstrated improved uptake relative to control MOP lacking c-RGD ligands. These results suggest a broad applicability of orthogonally functionalizable (covalent and non-covalent) MOPs in targeted drug delivery and imaging applications.