SEQUENCE-DEPENDENT VARIATION IN THE CONFORMATION OF DNA
SEQUENCE-DEPENDENT VARIATION IN THE CONFORMATION OF DNA
复制标题
DOI：
10.1016/0022-2836(81)90356-9
复制
发表时间：
1981-01-01
影响因子：
5.6
通讯作者：
KLUG, A
中科院分区：
文献类型：
--
作者：
LOMONOSSOFF, GP;BUTLER, PJG;KLUG, A
研究了DNA酶I对特定序列双链DNA聚合物的特异性作用。用交替共聚物poly d(A-T) .cntdot.poly d（A-T）和poly d(G-C) .cntdot.poly d（G-C）得到的结果支持了Klug等人（1979）的建议，即在生理盐条件下，含有交替嘌呤-嘧啶序列的双链DNA区域可能作为经典b型的结构变体存在。对含有交替dG-dC序列的确定低聚物的酶切表明，在类似条件下，这些低聚物在溶液中也以某种交替b结构存在。用低聚物获得的结果也为DNase I的作用模式提供了许多见解。在B-DNA十二聚体d（C-G-C-G-A-A-T-T-C-G-C-G）的情况下，晶体结构已经解决了（Dickerson和Drew， 1981），快速DNase I切割的位置与高局部螺旋扭转的位置之间存在非常好的相关性。
The specificity of action of the enzyme DNase I on double-stranded DNA polymers of defined sequence was investigated. The results obtained with the alternating copolymers poly d(A-T) .cntdot.poly d(A-T) and poly d(G-C) .cntdot.poly d(G-C) support the suggestion of Klug et al. (1979) that regions of double-stranded DNA containing alternating purine-pyrimidine sequences may exist as structural variants of the classical B-form under physiological salt conditions. Digestion of defined oligomers containing alternating dG-dC sequences indicate that these too exist in some alternating-B structure in solution under similar conditions. The results obtained with the oligomers also provide a number of insights into the mode of action of DNase I. In the case of the B-DNA dodecamer d(C-G-C-G-A-A-T-T-C-G-C-G), for which the crystal structure has been solved (Dickerson and Drew, 1981), there is a very good correlation between the sites of rapid DNase I cutting and positions of high local helical twist.