Global survey of alternative splicing and gene modules associated with fertility regulation in a thermosensitive genic male sterile wheat
Global survey of alternative splicing and gene modules associated with fertility regulation in a thermosensitive genic male sterile wheat
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与热敏基因雄性不育小麦育性调节相关的选择性剪接和基因模块的全球调查
DOI：
10.1093/jxb/erab516
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发表时间：
2021
影响因子：
6.9
通讯作者：
Liping Zhang
中科院分区：
文献类型：
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作者：
Jianfang Bai;Yukun Wang;Zihan Liu;Haoyu Guo;Fengting Zhang;Liping Guo;Shaohua Yuan;Wenjing Duan;Yanmei Li;Zhaoguo Tan;Changping Zhao;Liping Zhang
抽象。小麦温敏核不育系是两系杂交小麦的核心。了解温敏核不育小麦雄性不育的调控机制，有助于促进小麦育种。一些研究已经获得了关于在转录水平上与雄性不育相关的机制的信息，但尚不清楚选择性剪接的转录后过程如何有助于控制雄性不育。在这项研究中，我们进行了全基因组分析的选择性剪接在减数分裂阶段的温敏核不育系BS 366使用PacBio和RNA-Seq杂交测序。细胞学观察表明，BS 366在花粉细胞骨架组装、花粉和绒毡层细胞钙沉积以及绒毡层细胞小泡运输方面存在缺陷。根据我们的细胞学结果，49个差异剪接基因被分离出来。此外，25长的非编码RNA的目标和3 bHLH转录因子进行了鉴定。加权基因共表达网络分析检测到4个与BS 366结实率相关性较强的候选差异剪接基因，这些差异剪接基因是BS 366雄性不育性的直接表征。在这项研究中，我们获得了全面的数据关于选择性剪接介导的调节雄性不育在温敏核不育小麦。所确定的候选者可能为更好地理解雄性不育提供分子基础。
Abstract. Thermosensitive genic male sterile (TGMS) wheat lines are the core of two-line hybrid systems. Understanding the mechanism that regulates male sterility in TGMS wheat lines is helpful for promoting wheat breeding. Several studies have obtained information regarding the mechanisms associated with male sterility at the transcriptional level, but it is not clear how the post-transcriptional process of alternative splicing might contribute to controlling male sterility. In this study, we performed genome-wide analyses of alternative splicing during the meiosis stage in TGMS line BS366 using PacBio and RNA-Seq hybrid sequencing. Cytological observations indicated that cytoskeleton assembly in pollen cells, calcium deposition in pollen and tapetal cells, and vesicle transport in tapetal cells were deficient in BS366. According to our cytological findings, 49 differentially spliced genes were isolated. Moreover, 25 long non-coding RNA targets and three bHLH transcription factors were identified. Weighted gene co-expression network analysis detected four candidate differentially spliced genes that had strong co-relation with the seed setting percentage, which is the direct representation of male sterility in BS366. In this study, we obtained comprehensive data regarding the alternative splicing-mediated regulation of male sterility in TGMS wheat. The candidates identified may provide the molecular basis for an improved understanding of male sterility.