Contribution of solid-phase hexapeptide ligand libraries to the repertoire of human bile proteins
Contribution of solid-phase hexapeptide ligand libraries to the repertoire of human bile proteins
复制标题
DOI：
10.1016/j.chroma.2007.11.007
复制
发表时间：
2007-12-28
影响因子：
4.1
通讯作者：
Housset, C.
中科院分区：
文献类型：
--
作者：
Guerrier, L.;Claverol, S.;Housset, C.
胆汁中的蛋白质可能具有重要的生理功能，并可作为疾病的生物标志物。在这里，使用一种最近描述的类似色谱的技术分析了人类胆囊胆汁的蛋白质组成，这种技术能够增强低丰度物种的信号。首先，用固定肽配体文库处理胆汁液中的蛋白质，浓缩稀释和非常稀释的物种，同时稀释高丰度的蛋白质。通过迷你制备凝胶电泳对蛋白质进行经典分离后，使用LC-MS/MS对所得蛋白质混合物进行分析。共发现222个基因产物；其中143例未在蛋白质组学研究中报道。配体文库本身有助于发现分布在不同类别的81个新基因产物。所描述的色谱方法为胆汁蛋白库提供了重要的贡献，并为发现特定胆道疾病的标志物开辟了新的视角。(c) 2007 Elsevier B.V.版权所有
Proteins in bile may have important physiological functions and serve as disease biomarkers. Here, the protein composition of human gallbladder bile was analyzed using a recently described chromatography-like technology capable to enhance the signal of low-abundance species. First, proteins present in bile fluid were treated with immobilized peptide ligand libraries to concentrate dilute and very dilute species while concomitantly diluting the hi-h-abundance proteins. The analysis of resulting protein mixture was then performed using LC-MS/MS after having classically separated proteins by a mini preparative gel electrophoresis. Overall 222 gene products were found; 143 of them were not reported before in proteomics studies. Ligand libraries by themselves contributed to find 81 new gene products distributed throughout different categories. The described chromatographic approach provides a significant contribution to the bile protein repertoire and opens new perspectives for the discovery of markers for specific biliary tract diseases. (c) 2007 Elsevier B.V. All rights reserved.