Nature of the Sendai virus receptor: glycoprotein versus ganglioside
Nature of the Sendai virus receptor: glycoprotein versus ganglioside
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仙台病毒受体的性质：糖蛋白与神经节苷脂
DOI：
10.1128/jvi.33.1.304-310.1980
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发表时间：
1980
影响因子：
5.4
通讯作者：
Y. Isaacson
中科院分区：
文献类型：
--
作者：
Po;R. Ledeen;S. Udem;Y. Isaacson
神经节苷脂与糖蛋白作为仙台病毒的潜在受体进行了比较，通过将测定量的糖缀合物掺入卵磷脂-胆固醇脂质体中，并通过与绵羊红细胞的血凝试验测量结合。HeLa细胞神经节苷脂显示没有对病毒的结合活性高达15微克的唾液酸每5 μ mol的卵磷脂胆固醇，而HeLa细胞糖蛋白纳入类似的脂质体引起贪婪的病毒结合低于1微克的唾液酸。这些唾液酸糖蛋白可以通过多次氯仿-甲醇提取从大量的细胞蛋白中分离出来。纯化的大鼠脑神经节苷脂在120微克的唾液酸在脂质体的水平不结合病毒，而氯仿-甲醇提取的大鼠脑蛋白质只引起边缘结合。牛脑神经节苷脂与大鼠脑混合物略有不同，表现出弱结合特性。因此，我们的结果表明糖蛋白而不是神经节苷脂是仙台病毒的天然受体，并且组织中此类蛋白受体的数量不同。
Gangliosides were compared with glycoproteins as potential receptors for Sendai virus by incorporating measured amounts of the glycoconjugates into lecithin-cholesterol liposomes and measuring binding by a hemagglutination assay with sheep erythrocytes. HeLa cell gangliosides showed no binding activity toward the virus up to 15 micrograms of sialic acid per 5 mumol of lecithin-cholesterol, whereas HeLa cell glycoproteins incorporated into similar liposomes caused avid virus binding below 1 microgram of sialic acid. These sialoglycoproteins could be separated from the bulk of cell proteins by multiple chloroform-methanol extractions. Purified rat brain gangliosides at a level of 120 micrograms of sialic acid in liposomes did not bind virus, whereas chloroform-methanol-extracted rat brain proteins caused only marginal binding. Bovine brain gangliosides differed slightly from the rat brain mixture in showing weak binding properties. Our results thus indicate that glycoproteins, rather than gangliosides, are the natural receptors for Sendai virus and that tissues differ as to the quantity of such protein receptors.