Design of Multistimuli Responsive Hydrogels Using Integrated Modeling and Genetically Engineered Silk-Elastin-Like Proteins.
Design of Multistimuli Responsive Hydrogels Using Integrated Modeling and Genetically Engineered Silk-Elastin-Like Proteins.
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DOI：
10.1002/adfm.201600236
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发表时间：
2016-06-20
影响因子：
19
通讯作者：
Kaplan DL
中科院分区：
文献类型：
--
作者：
Huang W;Tarakanova A;Dinjaski N;Wang Q;Xia X;Chen Y;Wong JY;Buehler MJ;Kaplan DL
弹性的、坚固的和生物相容的水凝胶是罕见的，而在生物医学相关用途中对这些类型的生物材料的需求仍然很高。在这里，一个新的家庭的基因工程丝弹性蛋白共聚物（SELP）与编码酶交联位点的开发，为新一代的刺激响应，但强大的水凝胶。设计的输入由模拟指导，并通过遗传工程策略实现。在凝胶制造过程中避免γ辐射或化学交联，代替酶促过程，扩展了这些新凝胶用于掺入不稳定蛋白质和细胞的多功能性。在本研究中，新的SELP水凝胶提供了序列依赖性，可逆的刺激响应功能。它们的硬度几乎涵盖了软组织弹性的全部范围。此外，丝域的物理改性提供了一个次要的控制点，以微调机械刚度，同时保留刺激响应的功能，与各种生物医学材料和设备的需求的影响。
Elastomeric, robust, and biocompatible hydrogels are rare, while the need for these types of biomaterials in biomedical-related uses remains high. Here, a new family of genetically engineered silk-elastin copolymers (SELPs) with encoded enzymatic crosslinking sites is developed for a new generation of stimuli-responsive yet robust hydrogels. Input into the designs is guided by simulation, and realized via genetic engineering strategies. The avoidance of gamma irradiation or chemical crosslinking during gel fabrication, in lieu of an enzymatic process, expands the versatility of these new gels for the incorporation of labile proteins and cells. In the present study, the new SELP hydrogels offers sequence dependent, reversible stimuli-responsive features. Their stiffness covers almost the full range of the elasticity of soft tissues. Further, physical modification of the silk domains provided a secondary control point to fine-tune mechanical stiffness while preserving stimuli-responsive features, with implications for a variety of biomedical materials and device needs.