StarD13 3'-untranslated region functions as a ceRNA for TP53INP1 in prohibiting migration and invasion of breast cancer cells by regulating miR-125b activity
StarD13 3'-untranslated region functions as a ceRNA for TP53INP1 in prohibiting migration and invasion of breast cancer cells by regulating miR-125b activity
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StarD13 3'-非翻译区作为 TP53INP1 的 ceRNA，通过调节 miR-125b 活性来抑制乳腺癌细胞的迁移和侵袭。
DOI：
10.1016/j.ejcb.2017.11.002
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发表时间：
2018-01-01
影响因子：
6.6
通讯作者：
Xi, Tao
中科院分区：
文献类型：
--
作者：
Zheng, Lufeng;Li, Xiaoman;Xi, Tao
竞争性内源性信使RNA(CerNA)通过竞争性结合普通的microRNAs来影响其他RNA分子的转录。以往的研究表明，TP53INP1在肿瘤转移中起抑制作用。我们的研究阐明了StarD13信使RNA是调控乳腺癌细胞迁移和侵袭的CENA。经鉴定，microRNA-125b可诱导MCF-7细胞发生转移，并与StarD133‘UTR和TP53INP1 3’UTR结合。因此，StarD13和TP53INP1之间的Cerna相互作用是通过与miR-125b竞争结合而介导的。重要的是，StarD13上4546-4560nt的microRNA-125b结合位点对这种Cerna相互作用更重要。进一步证实了SPARC通过与TP53INP1竞争结合间接调控StarD13诱导乳腺癌细胞转移。总之，我们的发现证明了CENA调控网络可以更好地理解乳腺癌的转移机制。
Competitive endogenous messenger RNA (ceRNA) affects transcription of other RNA molecules by competitively binding common microRNAs. Previous studies have shown that TP53INP1 functions as a suppressor in tumor metastasis. Our study elucidated StarD13 messenger RNA as a ceRNA in regulating migration and invasion of breast cancer cells. MicroRNA-125b was identified to induce metastasis of MCF-7 cells and bind with both StarD13 3'UTR and TP53INP1 3'UTR. Therefore, a ceRNA interaction between StarD13 and TP53INP1 mediated by competitively binding to miR-125b was indicated. Importantly, a microRNA-125b binding site at 4546-4560 nt on StarD13 was verified more vital for this ceRNA interaction. Indirectly regulation of SPARC in inducing metastasis of breast cancer cells by StarD13 via competitively binding with TP53INP1 was further confirmed. In conclusion, our findings demonstrate a ceRNA regulatory network which could give a better understanding of metastatic mechanisms of breast cancer.