A RAPID METHOD FOR VIABLE CELL TITRATION AND CLONE PRODUCTION WITH HELA CELLS IN TISSUE CULTURE - THE USE OF X-IRRADIATED CELLS TO SUPPLY CONDITIONING FACTORS
A RAPID METHOD FOR VIABLE CELL TITRATION AND CLONE PRODUCTION WITH HELA CELLS IN TISSUE CULTURE - THE USE OF X-IRRADIATED CELLS TO SUPPLY CONDITIONING FACTORS
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DOI：
10.1073/pnas.41.7.432
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发表时间：
1955-01-01
影响因子：
11.1
通讯作者：
MARCUS, PI
中科院分区：
文献类型：
--
作者：
PUCK, TT;MARCUS, PI
描述了一种从HeLa群体的几乎所有细胞产生克隆的简单方法。该技术可用于滴定能够在悬浮液中繁殖的细胞的数量，并应在涉及病毒、药物、营养应激和诱变剂对动物细胞的作用的遗传和代谢研究中找到应用。
A simple method for production of clones from almost all the cells of HeLa population is described. The technique can be used to titrate the number of cells capable of reproduction in a suspension and should find application in genetic and metabolic studies involving the action on animal cells of viruses, drugs, nutritional stresses, and mutagenic agents.