Estimation of berberine in ayurvedic formulations containing Berberis aristata.
Estimation of berberine in ayurvedic formulations containing Berberis aristata.
复制标题
含有小檗的阿育吠陀配方中小檗碱的估计。
DOI：
10.1093/jaoac/91.5.1149
复制
发表时间：
2008
影响因子：
1.6
通讯作者：
S. Mishra
中科院分区：
文献类型：
--
作者：
K. K. Rout;Subhalaxmi Pradhan;S. Mishra
一种灵敏，简单，快速，高效的高效薄层色谱（HPTLC）方法已开发和验证的分析小檗碱在市售阿育吠陀制剂含有小檗DC监管的目的。在0.2 mm层厚的硅胶60 F254铝背TLC板上对这些制剂的甲醇提取物进行色谱分析。在33 +/-5 ° C下，用三元流动相丁醇-乙酸-水（8 + 1 + 1，v/v/v）将板显影至66 mm，罐饱和5分钟。标记物小檗碱在其最大吸光度350 nm处定量。发现检测限和定量限值为5和10 ng/斑点。校准图的线性回归分析数据显示，在小檗碱浓度为10 - 50 ng/斑点（相对于峰面积）的范围内，线性关系良好，相关系数= 0.9994。仪器精密度的变异系数（CV）为0.49%，方法的重复性为0.73% CV。98.27 - 99.11%的回收率值表明该方法具有良好的准确度。所开发的HPTLC方法是非常准确，精密度和成本效益，它已成功地应用于含有B的上市制剂的含量测定。测定小蘖碱的含量。
A sensitive, simple, rapid, and efficient high-performance thin-layer chromatographic (HPTLC) method has been developed and validated for the analysis of berberine in marketed Ayurvedic formulations containing Berberis aristata DC for regulatory purposes. Chromatography of methanolic extracts of these formulations was performed on silica gel 60 F254 aluminum-backed TLC plates of 0.2 mm layer thickness. The plate was developed up to 66 mm with the ternary-mobile phase butanol-acetic acid-water (8 + 1 + 1, v/v/v) at 33 +/- 5 degrees C with 5 min of tank saturation. The marker, berberine, was quantified at its maximum absorbance of 350 nm. The limit of detection and limit of quantitation values were found to be 5 and 10 ng/spot. The linear regression analysis data for the calibration plot showed a good linear relationship with correlation coefficient = 0.9994 in the concentration range of 10 to 50 ng/spot for berberine with respect to peak area. The instrumental precision was found to be 0.49% coefficient of variation (CV), and repeatability of the method was 0.73% CV. Recovery values from 98.27 to 99.11% indicate excellent accuracy of the method. The developed HPTLC method is very accurate, precise, and cost-effective, and it has been successfully applied to the assay of marketed formulations containing B. aristata for determination of berberine.