Expression and Distribution of Cystic Fibrosis Transmembrane Conductance Regulator in Neurons of the Human Brain
Expression and Distribution of Cystic Fibrosis Transmembrane Conductance Regulator in Neurons of the Human Brain
复制标题
囊性纤维化跨膜电导调节因子在人脑神经元中的表达和分布
DOI：
10.1369/jhc.2009.953455
复制
发表时间：
2009-12-01
影响因子：
3.2
通讯作者：
Gu, Jiang
中科院分区：
文献类型：
--
作者：
Guo, Yong;Su, Min;Gu, Jiang
分子囊性纤维化跨膜传导调节剂（CFTR）的重要性反映在其调节的许多生理功能上。已知存在于肺、胰腺、汗腺、肠道等组织的上皮细胞中，CFTR基因突变可引起囊性纤维化（CF）。采用逆转录聚合酶链反应、原位杂交和免疫组化等方法研究CFTR在人脑中的表达和分布。本研究证实了CFTR在人脑神经元中广泛而丰富的表达。连续组织切片的双重标记和评估技术将CFTR蛋白和mRNA信号定位到大脑所有区域的神经元细胞质中，但不定位到胶质细胞。CFTR在中枢神经元的存在，不仅为CF患者观察到的神经症状提供了可能的解释，而且可能有助于更好地了解CFTR在人脑中的功能。该手稿包含在线补充材料http://www.jhc.org。请在线访问本文以查看这些材料。
The importance of the molecule cystic fibrosis transmembrane conductance regulator (CFTR) is reflected in the many physiological functions it regulates. It is known to be present in epithelial cells of the lungs, pancreas, sweat glands, gut, and other tissues, and gene mutations of CFTR cause cystic fibrosis (CF). We studied the expression and distribution of CFTR in the human brain with reverse transcriptase polymerase chain reaction, in situ hybridization, and immunohistochemistry. This study demonstrates widespread and abundant expression of CFTR in neurons of the human brain. Techniques of double labeling and evaluation of consecutive tissue sections localized CFTR protein and mRNA signals to the cytoplasm of neurons in all regions of the brain studied, but not to glial cells. The presence of CFTR in central neurons not only provides a possible explanation for the neural symptoms observed in CF patients, but also may lead to a better understanding of the functions of CFTR in the human brain. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials.