NATURAL-KILLER-CELL ACTIVITY UNDER CONDITIONS REFLECTIVE OF TUMOR MICROENVIRONMENT
NATURAL-KILLER-CELL ACTIVITY UNDER CONDITIONS REFLECTIVE OF TUMOR MICROENVIRONMENT
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DOI：
10.1002/ijc.2910480617
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发表时间：
1991-07-30
影响因子：
6.4
通讯作者：
HEPPNER, GH
中科院分区：
文献类型：
--
作者：
LOEFFLER, DA;JUNEAU, PL;HEPPNER, GH
在低氧分压、低葡萄糖浓度和酸性pH条件下检测自然杀伤细胞(NK)活性，以确定肿瘤微环境中的物理条件是否能下调具有NK表型的肿瘤浸润性淋巴细胞的杀伤活性。低氧(0%O2)可显著降低NK活性，而低氧(1%O2)则不显著，酸性pH(6.4或6.7)对NK活性也有影响。低糖浓度(6 mg/dl)对NK活性无明显影响。中度改变(1%O2，26 mg/dl葡萄糖，pH 6.7)或极端改变(0%O2，6 mg/dl葡萄糖，pH 6.4)的组合显著降低NK活性。这项研究表明，实体瘤中存在的物理化学条件能够下调NK活性。
Natural-killer(NK) activity was examined in the presence of low oxygen tension, low glucose concentration and acidic pH, to determine whether physical conditions present in the tumor micro-environment could play a role in down-regulating cytolytic activity of tumor-infiltrating lymphocytes with NK phenotype. Anoxia (0% O2), but not hypoxia (1% O2), significantly reduced NK activity, as did acidic pH (6.4 or 6.7). Low glucose concentration (6 mg/dl) did not impair NK activity. Combinations of either moderate (1% O2, 26 mg/dl glucose, pH 6.7) or extreme (0% O2, 6 mg/dl glucose, pH 6.4) alteration of physical conditions significantly reduced NK activity. This study indicates that the physico-chemical conditions present within solid tumors are capable of down-regulating NK activity.