Decrease of the D4 dopamine receptor messenger RNA expression in lymphocytes from patients with major depression
Decrease of the D4 dopamine receptor messenger RNA expression in lymphocytes from patients with major depression
复制标题
重度抑郁症患者淋巴细胞中 D4 多巴胺受体信使 RNA 表达减少
DOI：
10.1016/s0278-5846(02)00253-1
复制
发表时间：
2002
影响因子：
5.6
通讯作者：
F. Bogetto
中科院分区：
文献类型：
--
作者：
P. Rocca;C. D. Leo;C. Eva;Livio Marchiaro;A. M. Milani;R. Musso;L. Ravizza;E. Zanalda;F. Bogetto
目的 由于工具相对不足，对多巴胺在精神疾病中可能作用的评估受到限制。检查重度抑郁症中多巴胺能系统变化的一个诱人方法是检查外周血单核细胞 (PBMC) 中多巴胺受体的表达。方法在 20-50 mg/天的帕罗西汀治疗 8 周之前和之后，检查 12 名重度抑郁症患者的 PBMC 中 D4 多巴胺受体 (D4DR) 信使 RNA (mRNA) 的表达。对十名健康受试者进行平行分析。以β-肌动蛋白为内标，通过逆转录聚合酶链式反应（RT-PCR）测定D4DR mRNA的相对含量。结果与对照组相比，未经治疗的抑郁症患者的D4DR mRNA水平显着降低。帕罗西汀治疗后，当患者的抑郁症状得到显着改善时，D4DR mRNA 表达恢复到控制水平。结论我们的研究结果表明，PBMC D4DR mRNA 表达作为重度抑郁症中枢多巴胺能功能的外周标志物的作用。
ObjectivesThe evaluation of the possible role of dopamine in psychiatric disorders has been limited by the relative inadequacy of tools. A tempting approach to examine alterations of dopaminergic system in major depression is to examine the expression of dopamine receptors in peripheral blood mononuclear cells (PBMC).MethodsD4dopamine receptor (D4DR) messenger RNA (mRNA) expression in PBMC from 12 patients with major depressive disorder was examined before and after an 8-week treatment with paroxetine at 20–50 mg/day. Ten healthy subjects were analyzed in parallel. The relative content of D4DR mRNA was determined by reverse transcriptase-polymerase chain reaction (RT-PCR), using β-actin as internal standard.ResultsD4DR mRNA levels were significantly decreased in untreated depressed patients as compared to controls. D4DR mRNA expression returned to control levels after paroxetine treatment, when patients achieved a significant improvement of depressive symptoms.ConclusionsResults of our study suggest the role of PBMC D4DR mRNA expression as a peripheral marker of the central dopaminergic function in major depression.
在正常人受试者中用[11C]雷氯必利和 PET 测量多巴胺的血清素能调节。
DOI：
10.1176/ajp.154.4.490
发表时间：
1997
期刊：
The American journal of psychiatry
影响因子：
--
作者：
Smith,GS;Dewey,SL;Brodie,JD;Logan,J;Vitkun,SA;Simkowitz,P;Schloesser,R;Alexoff,DA;Hurley,A;Cooper,T;Volkow,ND
通讯作者：
Volkow,ND