Dietary supplementation with polypeptides improved growth performance, antibacterial immune and intestinal microbiota structure of Litopenaeus vannamei
Dietary supplementation with polypeptides improved growth performance, antibacterial immune and intestinal microbiota structure of Litopenaeus vannamei
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膳食补充多肽可改善凡纳滨对虾的生长性能、抗菌免疫和肠道菌群结构
DOI：
10.1016/j.fsi.2019.06.033
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发表时间：
2019-09-01
影响因子：
4.7
通讯作者：
Zhang, Shuang
中科院分区：
文献类型：
--
作者：
Liao, Xu-zheng;Hu, Shi-kang;Zhang, Shuang
抗菌肽有望取代部分或全部抗生素，成为一种新型的饲料添加剂。但由于AMPS的制备成本高、作用机理不清楚等原因，限制了其应用。研究了以AMPS为主要成分的多肽（Polypeptide 5100）对凡纳滨对虾（Litopenaeus vannamei）生长、抗菌免疫和肠道微生物的影响。L.试验组在基础日粮中分别添加0.5%和1%的多肽5100，对照组在基础日粮中添加多肽5100，试验期为123 d。在饲料中添加1%的多肽5100可显著提高乳杆菌的增重率和比生长率。瓦纳梅。L.当感染哈维氏弧菌而非副溶血性弧菌时，0.5%和1%多肽S100组的凡纳滨对虾的生长率显著高于对照组。两个试验组的总超氧化物歧化酶（T-SOD）和溶菌酶（LZM）活性均显著高于对照组。0.5%多肽5100组的淀粉酶（AMS）和脂酶（LPS）活性显著高于对照组，而1.0%多肽S100组则显著低于对照组。Illumina MiSeq高通量测序结果显示，肠内优势菌门为L.凡纳滨对虾为变形菌门，其次为放射菌门、拟杆菌门、绿球藻门、蓝细菌门、梭杆菌门和细球藻门，实验组优势门蓝细菌丰度明显上调。在科水平上，在1.0%多肽5100组中观察到假单胞菌科和黄单胞菌科显著增加，但弧菌科减少。两个实验组的优势菌属发光杆菌的丰度均明显下降。不太可能，假单胞菌属和寡养单胞菌属的丰度在1.0%多肽S100组中明显增加，但在0.5%多肽5100组中与对照无显著差异。这些结果表明，多肽S100可以改善乳酸菌的生长性能、抗菌免疫和肠道菌群结构。瓦纳梅。
Antibacterial peptides (AMPS) are expected to replace some or all of the antibiotics and become a new feed additive. However, the high production cost and unclear mechanism limited the application of AMPS. In this research, the effects of a commercial polypeptide (Polypeptide 5100) whose main components are AMPS on the growth, antibacterial immune and intestinal microbial of Litopenaeus vannamei were study. L. vannamei (initial weight of 0.16 +/- 0.03 g) were fed for 123 days with basal diet added Polypeptide 5100 at two levels each (0.5% and 1%) as experimental groups, and a basal diet as control. Dietary inclusion of Polypeptide 5100 at 1% level significantly increased the weight gain (WG) and specific growth rate (SGR) of L. vannamei. The survival rates of L. vannamei in 0.5% and 1% Polypeptide S100 groups were significantly higher than the control when infected by Vibrio harveyi but not Vibrio parahaemolyticus. The activities of total superoxide dismutase (T-SOD) and lysozyme (LZM) in the two experimental groups were all significantly higher than the control. Differently, the activities of amylase (AMS) and lipase (LPS) were significantly higher in 0.5% Polypeptide 5100 group but lower in 1.0% Polypeptide S100 group. Illumina MiSeq high-throughput sequencing showed that the dominant phyla in the intestine of L. vannamei were Proteobacteria, followed by Actinobacteria, Bacteroidetes, Chloroflexi, Cyanobacteria, Fusobacteria and Tenericutes, and the abundance of predominant phyla Cyanobacteria were upregulated significantly in the experimental groups. At the family level, significant increase was observed in Pseudomonadaceae and Xanthomonadaceae but decrease in Vibrionaceae in the 1.0% Polypeptide 5100 group. The abundance of predominant genus Photobacteterium were obviously downregulated in the two experimental groups. Unlikely, the abundance of Pseudomonas and Stenotrophomonas were distinctly increased in the 1.0% Polypeptide S100 group but not significantly different from the control in 0.5% Polypeptide 5100 group. All these results suggested that Polypeptide S100 could improve the growth performance, antibacterial immune and intestinal microbiota structure of L. vannamei.