High-grade transgenic somatic chimeras from chicken embryonic stem cells
High-grade transgenic somatic chimeras from chicken embryonic stem cells
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DOI：
10.1016/j.mod.2005.10.002
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发表时间：
2006-01-01
影响因子：
2.6
通讯作者：
Etches, RJ
中科院分区：
文献类型：
--
作者：
van de Lavoir, MC;Mather-Love, C;Etches, RJ
雄性和雌性胚胎干 (ES) 细胞系源自 X 阶段 (EG&K) 鸡胚胎的透明区域。这些 ES 细胞系在培养物中生长较长时间，并且大多数细胞保留二倍体核型。当重新引入 VI-X 阶段 (EG&K) 受体胚胎时，cES 细胞能够分化为所有体细胞组织。通过将受体胚胎的照射与将 cES 细胞暴露于滞育胚胎环境相结合，获得了高频率和高程度的嵌合。产生了高级嵌合体，通过羽毛色素沉着与供体表型无法区分。编码 GFP 的转基因在普遍存在的启动子 CX 的控制下被整合到 cES 细胞的基因组中，并且 GFP 在体细胞组织中广泛表达。尽管cES细胞对体细胞组织做出了广泛的贡献，但没有观察到对种系的贡献。 (c) 2005 Elsevier Ireland Ltd. 保留所有权利。
Male and female embryonic stem (ES) cell lines were derived from the area pellucidae of Stage X (EG&K) chicken embryos. These ES cell lines were grown in culture for extended periods of time and the majority of the cells retained a diploid karyotype. When reintroduced into Stage VI-X (EG&K) recipient embryos, the cES cells were able to contribute to all somatic tissues. By combining irradiation of the recipient embryo with exposure of the cES cells to the embryonic environment in diapause, a high frequency and extent of chimerism was obtained. High-grade chimeras, indistinguishable from the donor phenotype by feather pigmentation, were produced. A transgene encoding GFP was incorporated into the genome of cES cells under control of the ubiquitous promoter CX and GFP was widely expressed in somatic tissues. Although cES cells made extensive contributions to the somatic tissues, contribution to the germline was not observed. (c) 2005 Elsevier Ireland Ltd. All rights reserved.