Evaluating Different Fixation Protocols for Spectral Cytopathology, Part 1
Evaluating Different Fixation Protocols for Spectral Cytopathology, Part 1
复制标题
DOI：
10.1021/ac202046d
复制
发表时间：
2012-02-07
影响因子：
7.4
通讯作者：
Diem, Max
中科院分区：
文献类型：
--
作者：
Mazur, Antonella I.;Marcsisin, Ellen J.;Diem, Max
光谱细胞病理学（SCP）是一种新的疾病诊断方法，它利用红外光谱来询问细胞样品的生化成分和多元统计方法，如主成分分析，来分析和诊断光谱。在过去的十年中，SCP在疾病诊断中的应用取得了长足的进步;然而，固定引起的变化和样品处理方法仍然没有得到系统的理解。相反，常规细胞病理学中的固定和染色方法，通常涉及维持细胞形态的方案，已经被记载并被广泛接受了近世纪。对于SCP，固定程序必须保留样品的生化组成，以便不掩盖对疾病诊断有意义的光谱变化。我们报告的努力，研究传统的细胞病理学和SCP中常用的固定协议的影响，包括固定和未固定的方法适用于脱落的口腔（颊）粘膜细胞。数据表明，在固定剂中的时间长度和通过干燥储存样品的持续时间有助于光谱几乎重叠的微小光谱变化。这些发现表明，与疾病引起的变化相比，固定影响的变化可以忽略不计。
Spectral cytopathology (SCP) is a novel approach for disease diagnosis that utilizes infrared spectroscopy to interrogate the biochemical components of cellular samples and multivariate statistical methods, such as principal component analysis, to analyze and diagnose spectra. SCP has taken vast strides in its application for disease diagnosis over the past decade; however, fixation-induced changes and sample handling methods are still not systematically understood. Conversely, fixation and staining methods in conventional cytopathology, typically involving protocols to maintain the morphology of cells, have been documented and widely accepted for nearly a century. For SCP, fixation procedures must preserve the biochemical composition of samples so that spectral changes significant to disease diagnosis are not masked. We report efforts to study the effects of fixation protocols commonly used in traditional cytopathology and SCP, including fixed and unfixed methods applied to exfoliated oral (buccal) mucosa cells. Data suggest that the length of time in fixative and duration of sample storage via desiccation contribute to minor spectral changes where spectra are nearly superimposable. These findings illustrate that changes influenced by fixation are negligible in comparison to changes induced by disease.