Antibacterial and Anti-Inflammatory pH-Responsive Tannic Acid-Carboxylated Agarose Composite Hydrogels for Wound Healing
Antibacterial and Anti-Inflammatory pH-Responsive Tannic Acid-Carboxylated Agarose Composite Hydrogels for Wound Healing
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DOI：
10.1021/acsami.6b10491
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发表时间：
2016-10-26
影响因子：
9.5
通讯作者：
Blencowe, Anton
中科院分区：
文献类型：
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作者：
Ninan, Neethu;Forget, Aurelien;Blencowe, Anton
PH敏感水凝胶在药物控释应用中发挥着重要作用，并有可能影响伤口的治疗。在这项研究中，我们报道了一种新型的锌离子交联的羧化琼脂/单宁酸水凝胶支架的制备，用于单宁酸的pH控制释放。所得水凝胶在中性和碱性pH下对单宁酸的释放可以忽略不计，在酸性pH下持续释放，在那里它们也表现出最大的溶胀。该水凝胶还表现出良好的抗菌和抗炎性能，对3T3成纤维细胞系无细胞毒性。在模拟创伤试验中，观察到接触单宁酸水凝胶提取物的细胞迁移和增殖显著增加。此外，单宁酸水凝胶能够以浓度依赖的方式抑制刺激的人巨噬细胞中NO的产生，表明其具有有效的抗炎活性。综上所述，这些新型pH敏感型水凝胶的细胞相容性、抗菌和抗炎特性使其有望成为伤口敷料的候选材料。
pH-sensitive hydrogels play an important role in controlled drug release applications and have the potential to impact the management of wounds. In this study, we report the fabrication of novel carboxylated agarose/tannic acid hydrogel scaffolds cross-linked with zinc ions for the pH-controlled release of tannic acid. The resulting hydrogels exhibited negligible release of tannic acid at neutral and alkaline pH and sustained release at acidic pH, where they also displayed maximum swelling. The hydrogels also displayed favorable antibacterial and anti-inflammatory properties, and a lack of cytotoxicity toward 3T3 fibroblast cell lines. In simulated wound assays, significantly greater cell migration and proliferation was observed for cells exposed to tannic acid hydrogel extracts. In addition, the tannic acid hydrogels were able to suppress NO production in stimulated human macrophages in a concentration-dependent manner, indicating effective anti-inflammatory activity. Taken together, the cytocompatibility, antibacterial, and anti-inflammatory characteristics of these novel pH-sensitive hydrogels make them promising candidates for wound dressings.