Human CD8 transgene regulation of HLA recognition by murine T cells.
Human CD8 transgene regulation of HLA recognition by murine T cells.
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鼠T细胞对HLA识别的人CD8转基因调节。
DOI：
10.1084/jem.182.5.1315
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发表时间：
1995-11-01
影响因子：
15.3
通讯作者：
Peterson, Per A.
中科院分区：
文献类型：
--
作者：
Laface, Drake M.;Vestberg, Mikael;Yang, Young;Srivastava, Rakesh;Disanto, Jim;Flomenberg, Neal;Brown, Shafeeka;Sherman, Linda A.;Peterson, Per A.
本研究建立了一系列在胸腺和外周表达差异的人CD 8转基因（hCD 8 Tg）小鼠，以研究CD 8辅助受体对库选择和T细胞应答的调节作用。hCD 8的表达显著增强了对HLA I类分子和杂合A2/Kb分子的应答，为α 3结构域之外的第二个相互作用位点提供了功能证据，该位点对于最佳辅助受体功能至关重要。hCD 8的外周T细胞表达足以增强对HLA I类的响应性，因为缺乏胸腺表达的hCD 8 Tg小鼠以及在胸腺和外周中表达hCD 8的小鼠响应。表达hCD 8转基因的鼠CD 8+和CD 4 + T细胞对外源HLA I类的反应均显著增强，揭示了在鼠I类或II类上选择的T细胞受体库识别人I类主要组织相容性复合体（MHC）的能力。与识别外源I类相反，胸腺表达hCD 8转基因绝对需要增强对自身HLA I类限制性抗原肽的识别。因此，我们的研究揭示了不同的要求CD 8辅助受体的表达在胸腺选择的T细胞库响应外来MHC和抗原肽结合到自身的MHC，提供了一个新的证明，是依赖于人类CD 8的积极选择。
A series of human CD8 transgenic (hCD8 Tg) mice with differential expression in the thymus and periphery were produced to investigate CD8 coreceptor regulation of repertoire selection and T cell responses. Expression of hCD8 markedly enhanced responses to both HLA class I molecules and hybrid A2/Kb molecules providing functional evidence for a second interaction site, outside of the alpha 3 domain, which is essential for optimal coreceptor function. Peripheral T cell expression of hCD8 was sufficient to augment responsiveness to HLA class I, as hCD8 Tg mice which lacked thymic expression responded as well as mice expressing hCD8 in the thymus and periphery. Both murine CD8+ and CD4+ T cells expressing hCD8 transgenes exhibited markedly enhanced responses to foreign HLA class I, revealing the ability of T cell receptor repertoires selected on either murine class I or class II to recognize human class I major histocompatibility complex (MHC). In contrast to recognition of foreign class I, thymic expression of hCD8 transgenes was absolutely required to enhance recognition of antigenic peptide restricted by self-HLA class I. Thus, our studies revealed disparate requirements for CD8 coreceptor expression in the thymus for selection of a T cell repertoire responsive to foreign MHC and to antigenic peptides bound to self-MHC, providing a novel demonstration of positive selection that is dependent on human CD8.
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DOI：
10.1084/jem.179.6.1997
发表时间：
1994-06-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Robey E;Itano A;Fanslow WC;Fowlkes BJ
通讯作者：
Fowlkes BJ
DOI：
10.1084/jem.166.4.956
发表时间：
1987-10-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Potter TA;Bluestone JA;Rajan TV
通讯作者：
Rajan TV
DOI：
10.1073/pnas.85.20.7690
发表时间：
1988-10-01
影响因子：
11.1
作者：
CHAMBERLAIN, JW;NOLAN, JA;WEISSMAN, SM
通讯作者：
WEISSMAN, SM
影响因子：
64.8
作者：
KALINKE, U;ARNOLD, B;HAMMERLING, GJ
通讯作者：
HAMMERLING, GJ
DOI：
10.1073/pnas.83.21.8298
发表时间：
1986-11-01
影响因子：
11.1
作者：
EMMRICH, F;STRITTMATTER, U;EICHMANN, K
通讯作者：
EICHMANN, K