Relationship between expression of p21WAF1/CIP1 and radioresistance in human gliomas.
Relationship between expression of p21WAF1/CIP1 and radioresistance in human gliomas.
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DOI：
10.1111/j.1349-7006.1999.tb00795.x
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发表时间：
1999-06
期刊：
影响因子：
--
通讯作者：
Tamaki N
中科院分区：
文献类型：
--
作者：
Kokunai T;Tamaki N
p21 WAF 1/CIP 1（p21）在γ射线照射后DNA修复和凋亡中的作用仍存在争议。在这项研究中，p21对人脑肿瘤放射敏感性的影响进行了研究。对切除的肿瘤进行p21的化学染色。p21在星形细胞瘤中表达较高，而在髓母细胞瘤、生殖细胞瘤和原发性恶性淋巴瘤中表达较低。用pcDNA/p21转染胶质瘤和髓母细胞瘤细胞系以引起p21过表达，然后在对转染和未转染的细胞进行γ射线照射后评估肿瘤细胞集落形成和凋亡。p21过表达增强了γ射线照射后p53蛋白缺陷或不缺陷的人脑肿瘤细胞系的克隆形成存活率并抑制了细胞凋亡。当p21在胶质瘤细胞系中过度产生时，无论p53状态如何，都获得了放射抗性。
The role of p21WAF1/CIP1 (p21) in DNA repair and apoptosis following γ‐irradiation remains controversial. In this study the influence of p21 on the radiosensitivity of human brain tumors was investigated. Resected tumors were stained immunohistochemically for p21. Expression of p21 in astrocytic tumors was high, but it was low in medulloblastomas, germinomas, and primary malignant lymphomas. Glioma and medulloblastoma cell lines were transfected with pcDNA/p21 to cause p21 overexpression, then tumor‐cell colony formation and apoptosis were assessed following γ‐irradiation of the transfected and nontransfected cells. Overexpression of p21 enhanced clono‐genic survival and suppressed apoptosis after γ‐irradiation in human brain tumor cell lines with or without p53 protein deficiency. Radioresistance was acquired when p21 was overproduced in the glioma cell lines irrespective of p53 status.
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影响因子：
64.8
作者：
LUO, Y;HURWITZ, J;MASSAGUE, J
通讯作者：
MASSAGUE, J
影响因子：
64.5
作者：
ELDEIRY, WS;TOKINO, T;VOGELSTEIN, B
通讯作者：
VOGELSTEIN, B
DOI：
10.1073/pnas.80.20.6361
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DUKE, RC;CHERVENAK, R;COHEN, JJ
通讯作者：
COHEN, JJ
影响因子：
8
作者：
Fan, SJ;Chang, JK;OConnor, PM
通讯作者：
OConnor, PM
影响因子：
8
作者：
Lu, YJ;Yamagishi, N;Takebe, H
通讯作者：
Takebe, H