Complementation of a Borrelia afzelii OspC mutant highlights the crucial role of OspC for dissemination of Borrelia afzelii in Ixodes ricinus
Complementation of a Borrelia afzelii OspC mutant highlights the crucial role of OspC for dissemination of Borrelia afzelii in Ixodes ricinus
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DOI：
10.1016/j.ijmm.2006.11.003
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发表时间：
2007-04-01
影响因子：
4.1
通讯作者：
Schulte-Spechtel, Ulrike
中科院分区：
文献类型：
--
作者：
Fingerle, Volker;Goettner, Gereon;Schulte-Spechtel, Ulrike
外表面蛋白（Osp）组成的改变-特别是OspA和OspC -似乎是重要的适应伯氏疏螺旋体广义上的吸热宿主（哺乳动物）和变温载体（蜱）。OspA可能介导粘附蜱中肠细胞，从而使疏螺旋体在载体中存活，而OspC与疏螺旋体侵入蜱唾液腺和感染哺乳动物宿主相关。在这里，我们描述了第一次成功的转化和互补的阿氏疏螺旋体ospC突变体与野生型ospC的反式。为了测试ospC在蜱传播行为的影响，未喂养的蓖麻硬蜱人工感染的毛细管喂养与阿氏疏螺旋体野生型，B。afzelii ospC突变体或ospC互补克隆。蜱的中肠和唾液腺进行了研究后，不同的时间间隔的存在疏螺旋体和OspA和OspC的单克隆抗体免疫荧光染色。而B.阿氏野生型菌株在其表面上表现出丰富的OspC，传播到唾液腺，OspC阴性突变体仅存在于蜱中肠中。组成型表达野生型ospC的ospC互补克隆再次能够定殖唾液腺。这一发现表明，OspC是至关重要的传播B。从蜱的中肠到唾液腺，这是感染温血宿主的先决条件。Goettner等人[2006年]已对此处提供的详细数据进行了总结。B的OspC。如欧洲OspC缺陷型B的转化和互补所示，afzelii对于载体中的传播至关重要。afzelii菌株国际医学微生物学杂志296 SI（Suppl. 40），122-124]。(c)2007年，Elsevier GmbH。All rights reserved.
Alteration of the outer surface protein (Osp) composition - especially that of OspA and OspC - seems to be important for the adaptation of Borrelia burgdorferi sensu lato to its endothermic hosts (mammals) and poikilothermic vectors (ticks). OspA possibly mediates adherence to tick midgut cells thus enabling the borreliae to survive in the vector, while OspC is associated with borrelial invasion of the tick salivary glands and infection of the mammalian hosts. Here we describe the first successful transformation and complementation of a Borrelia afzelii ospC mutant with the wild-type ospC in trans. To test the influence of OspC on the dissemination behavior in ticks, unfed Ixodes ricinus nymphs were artificially infected by capillary feeding either with R afzelii wild type, the B. afzelii ospC mutant or the ospC-complemented clone. Tick midguts and salivary glands were investigated after different time intervals for the presence of borreliae and for OspA and OspC by immunfluorescence staining with monoclonal antibodies. While the B. afzelii wild-type strain exhibiting abundant OspC on its surface disseminated to the salivary glands, the OspC-negative mutant was only present in the tick midguts. The ospC-complemented clone which constitutively expresses the wild-type ospC was again able to colonize the salivary glands. This finding demonstrates that OspC is crucial for dissemination of B. afzelii from the tick midgut to the salivary glands, a prerequisite for infection of the warm-blooded host. A summary of the detailed data presented here has already been given in Goettner et al. [2006. OspC of B. afzelii is crucial for dissemination in the vector as shown by transformation and complementation of a European OspC-deficient B. afzelii strain. Int. J. Med. Microbiol. 296SI(Suppl. 40), 122-124]. (c) 2007 Elsevier GmbH. All rights reserved.