Metagenome analyses of corroded concrete wastewater pipe biofilms reveal a complex microbial system.
Metagenome analyses of corroded concrete wastewater pipe biofilms reveal a complex microbial system.
复制标题
DOI：
10.1186/1471-2180-12-122
复制
发表时间：
2012-06-22
期刊：
影响因子：
4.2
通讯作者：
Santo Domingo JW
中科院分区：
文献类型：
--
作者：
Gomez-Alvarez V;Revetta RP;Santo Domingo JW
混凝土对污水收集系统的腐蚀是恶化和过早坍塌的重要原因。未能充分解决日益恶化的基础设施网络威胁着我们的环境、公共健康和安全。通过对全基因组焦糖测序数据和16S rRNA基因克隆文库的分析，确定了从一条腐蚀的污水管道的顶端(顶部)和反面(底部)采集的微生物组成和与生物量相关的功能基因。分类学和功能分析表明，大约90%的总多样性与放线杆菌门、拟杆菌门、菲尔米特菌门和变形杆菌门有关。顶管(TP)和底管(BP)群落组成不同，部分差异归因于硫化物氧化细菌和硫酸盐还原细菌的丰富。此外，人类粪便细菌在BP群落中更为丰富。在功能类别中，与硫和氮代谢有关的蛋白质在不同生物膜之间表现出最显著的差异。与重金属抗性、毒力(蛋白质分泌系统)和胁迫反应相关的基因在TP生物膜中也有丰富的表达，而在BP生物膜中发现了更多与运动和趋化相关的基因。这两种生物膜都包含大量与抗生素耐药性和有毒化合物子系统相关的基因。废水生物膜的功能潜力具有高度的多样性，COG的多样性水平与土壤的描述水平相似。根据元基因组数据，可能影响生态位分化的一些因素是pH、好氧条件和底物的有效性，如氮和硫。本研究的结果将有助于我们更好地了解废水混凝土生物膜中微生物成员的遗传网络和功能能力。
Concrete corrosion of wastewater collection systems is a significant cause of deterioration and premature collapse. Failure to adequately address the deteriorating infrastructure networks threatens our environment, public health, and safety. Analysis of whole-metagenome pyrosequencing data and 16S rRNA gene clone libraries was used to determine microbial composition and functional genes associated with biomass harvested from crown (top) and invert (bottom) sections of a corroded wastewater pipe. Taxonomic and functional analysis demonstrated that approximately 90% of the total diversity was associated with the phyla Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria. The top (TP) and bottom pipe (BP) communities were different in composition, with some of the differences attributed to the abundance of sulfide-oxidizing and sulfate-reducing bacteria. Additionally, human fecal bacteria were more abundant in the BP communities. Among the functional categories, proteins involved in sulfur and nitrogen metabolism showed the most significant differences between biofilms. There was also an enrichment of genes associated with heavy metal resistance, virulence (protein secretion systems) and stress response in the TP biofilm, while a higher number of genes related to motility and chemotaxis were identified in the BP biofilm. Both biofilms contain a high number of genes associated with resistance to antibiotics and toxic compounds subsystems. The function potential of wastewater biofilms was highly diverse with level of COG diversity similar to that described for soil. On the basis of the metagenomic data, some factors that may contribute to niche differentiation were pH, aerobic conditions and availability of substrate, such as nitrogen and sulfur. The results from this study will help us better understand the genetic network and functional capability of microbial members of wastewater concrete biofilms.