Campylobacter jejuni motility integrates specialized cell shape, flagellar filament, and motor, to coordinate action of its opposed flagella.
Campylobacter jejuni motility integrates specialized cell shape, flagellar filament, and motor, to coordinate action of its opposed flagella.
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DOI：
10.1371/journal.ppat.1008620
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发表时间：
2020-07-01
期刊：
影响因子：
6.7
通讯作者：
Beeby, Morgan
中科院分区：
文献类型：
--
作者：
Cohen, Eli J;Nakane, Daisuke;Beeby, Morgan
空肠弯曲杆菌在每根杆子上旋转一个鞭毛，游过宿主胃肠道粘稠的粘膜。然而，尽管它们对宿主的定殖很重要，但空肠弯曲杆菌如何协调这两个相对的鞭毛的旋转尚不清楚。除了它们的极地位置，空肠弯曲杆菌的鞭毛在其他方面也与肠杆菌科的正常情况不同：它们的鞭毛马达产生的扭矩要大得多，它们的鞭毛细丝由两个不同的鞭毛组成。为了了解空肠弯曲菌的相对马达是如何协调的，以及这些因素在空肠弯曲杆菌的运动中起到了什么作用，我们开发了可以被荧光标记的鞭毛菌株，并用高速视频显微镜观察了它们。我们发现，在高粘度介质中游泳时，空肠弯曲杆菌通过将前导纤维包裹在细胞体周围来协调其双鞭毛，其分化的鞭毛纤维和螺旋体已经进化为促进这种包裹模式的游泳。
Campylobacter jejuni rotates a flagellum at each pole to swim through the viscous mucosa of its hosts' gastrointestinal tracts. Despite their importance for host colonization, however, how C. jejuni coordinates rotation of these two opposing flagella is unclear. As well as their polar placement, C. jejuni's flagella deviate from the norm of Enterobacteriaceae in other ways: their flagellar motors produce much higher torque and their flagellar filament is made of two different zones of two different flagellins. To understand how C. jejuni's opposed motors coordinate, and what contribution these factors play in C. jejuni motility, we developed strains with flagella that could be fluorescently labeled, and observed them by high-speed video microscopy. We found that C. jejuni coordinates its dual flagella by wrapping the leading filament around the cell body during swimming in high-viscosity media and that its differentiated flagellar filament and helical body have evolved to facilitate this wrapped-mode swimming.