High frequency transcranial magnetic stimulation mimics the effects of ECS in upregulating astroglial gene expression in the murine CNS
High frequency transcranial magnetic stimulation mimics the effects of ECS in upregulating astroglial gene expression in the murine CNS
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DOI：
10.1016/s0169-328x(96)00232-x
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发表时间：
1997-03-01
期刊：
影响因子：
--
通讯作者：
Steward, O
中科院分区：
文献类型：
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作者：
Fujiki, M;Steward, O
本研究评估的后果，高频（25赫兹）经颅磁刺激的表达胶质细胞酸性蛋白（GFAP）在小鼠中枢神经系统。通过5 cm直径的圆形线圈向小鼠递送经颅磁刺激列（1-30列，25 Hz，10 s持续时间）。刺激产生刺激锁定运动反应，但没有引起行为癫痫发作。原位杂交法检测刺激后12、24、36、48 h、4 d和5 d GFAP mRNA水平。在多次25 Hz的训练后，海马齿状回中GFAP mRNA的水平显著增加;在大脑皮层中观察到更适度的增加。齿状回GFAP mRNA的选择性增加与单次电惊厥发作（ECS）后观察到的相似。这些结果表明，经颅磁刺激可用于调节星形胶质细胞的基因表达，诱导反应性反应的第一阶段，类似于神经系统损伤后发生的反应。
The present study evaluates the consequences of high frequency (25 hz) trans-cranial magnetic stimulation on the expression of glial fibrillary acidic protein (GFAP) in the murine CNS. Trains of transcranial magnetic stimulation (1-30 trains at 25 Hz, 10 s duration) were delivered to mice via 5-cm diameter round coils. The stimulation produced stimulus-locked motor responses but did not elicit behavioral seizures. GFAP mRNA levels were evaluated 12, 24, 36, 48 h, 4 days, and s days following stimulation by in situ hybridization. Following multiple 25 Hz trains, there were dramatic increases in the levels of GFAP mRNA in the hippocampal dentate gyrus; more modest increases were observed in the cerebral cortex. The selective increases in GFAP mRNA in the dentate gyrus were similar to those observed following single electroconvulsive seizures (ECS). These results indicate that trans-cranial magnetic stimulation can be used to modulate astroglial gene expression, inducing the first stage of a reactive response that is similar to what occurs following nervous system injury.