Are ruminal bacteria protected against environmental stress by plant antioxidants?
Are ruminal bacteria protected against environmental stress by plant antioxidants?
复制标题
植物抗氧化剂是否可以保护瘤胃细菌免受环境压力？
DOI：
10.1046/j.1472-765x.2002.01185.x
复制
发表时间：
2002
影响因子：
2.4
通讯作者：
P. Javorskỳ
中科院分区：
文献类型：
--
作者：
K. Holovská;V. Lenártová;K. Holovská;P. Pristaš;P. Javorskỳ
目的：研究了在植物抗氧化剂存在下，氯化汞（HgCl 2）暴露对瘤胃细菌牛链球菌抗氧化酶超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSHPx）活性的影响。
方法和结果：牛链球菌在0或5 μg ml−1的HgCl 2单独或与抗氧化物质（AOS）一起生长：硒代甲硫氨酸（Se），α-生育酚（αtoc），β-胡萝卜素（βcar），褪黑素（mel）。 测定了菌体破碎上清液中SOD和GHPx的活性。链球菌的显著减少。在HgCl_2和AOS存在下，观察牛SOD活性。链球菌GSHPx活性牛的GSHPx活性无明显变化，只有在βcar存在时才显著降低。
结论：硒、αtoc和βcar对消除汞诱发的瘤胃链球菌环境应激有积极作用。牛的体外试验记录。
本研究的意义和影响：探讨了植物抗氧化剂在消除重金属引起的瘤胃细菌环境胁迫中的潜在作用。
Aims: To investigate the activity response of the antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GSHPx) of the rumen bacterium Streptococcus bovis following exposure to mercury(II) chloride (HgCl2) in the presence of plant antioxidants.
Methods and Results:Streptococcus bovis was grown with 0 or 5 μg ml−1 of HgCl2 alone or together with antioxidant substances (AOS): seleno‐l‐methionine (Se), α‐tocopherol (αtoc), β‐carotene (βcar), melatonin (mel). The activities of SOD and GHPx were estimated in supernatants of disrupted bacterial cells. A significant decrease in the Strep. bovis SOD activity in the presence of HgCl2 and tested AOS, except mel, was observed. The GSHPx activity of Strep. bovis was under the same cultivation conditions nonsignificantly changed and a significant decrease in the GSHPx activity was recorded only in the presence of βcar.
Conclusions: The positive effect of Se, αtoc and βcar on the elimination of environmental stress, evoked by mercury, in ruminal bacterium Strep. bovis in vitro was documented.
Significance and Impact of the Study: The potential role of plant antioxidants in elimination of the environmental stress of ruminal bacteria evoked by heavy metals is discussed.