IgE antibody repertoire in nasal secretions of children and adults with seasonal allergic rhinitis: A molecular analysis
IgE antibody repertoire in nasal secretions of children and adults with seasonal allergic rhinitis: A molecular analysis
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DOI：
10.1111/pai.13148
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发表时间：
2020-01-22
影响因子：
4.4
通讯作者：
Matricardi, Paolo Maria
中科院分区：
文献类型：
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作者：
Castelli, Sveva;Arasi, Stefania;Matricardi, Paolo Maria
背景：鼻分泌物IgE检测和季节性变应性鼻炎分子诊断的研究日益受到重视。然而，鼻IgE检测与最新的分子检测的可靠性从来没有在一个大的花粉过敏患者队列进行评估。目的应用基因芯片技术研究并比较大量儿童和成人SAR患者血清及NS中特异性IgE（sIgE）抗体谱。方法采用美敦力Merocel 2000（R）吸收装置和最小稀释程序收集90名儿童和71名成人SAR的鼻分泌物。在NS和血清中测量总IgE（tIgE）（ImmunoCAP，Thermo Fisher Scientific（TFS））和针对112种过敏原分子的sIgE抗体（ISAC-112，TFS）。结果68.3%的患者鼻粘膜sIgE阳性。鼻内sIgE抗体识别空气（88%）、蔬菜（10%）和动物性食物或其他（
Background There is growing interest both in testing IgE in nasal secretions (NS) and in molecular diagnosis of seasonal allergic rhinitis (SAR). Yet, the reliability of nasal IgE detection with the newest molecular assays has never been assessed in a large cohort of pollen allergic patients. Objective To investigate with microarray technology and compare the repertoires of specific IgE (sIgE) antibodies in NS and sera of a large population of children and adults with SAR. Methods Nasal secretions were collected with an absorbent device (Merocel 2000 (R), Medtronic) and a minimal dilution procedure from 90 children and 71 adults with SAR. Total IgE (tIgE) (ImmunoCAP, Thermo Fisher Scientific (TFS)) and sIgE antibodies against 112 allergen molecules (ISAC-112, TFS) were measured in NS and serum. Results Nasal sIgE was detectable in 68.3% of the patients. The detected nasal sIgE antibodies recognized airborne (88%), vegetable (10%), and animal food or other (